Laboratory "Health Systemic Process", EA4129, University Lyon 1, 69008 Lyon, France.
Virology Laboratory, Institute of Infectious Agents, Croix-Rousse Hospital, Hospices Civils of Lyon, 69004 Lyon, France.
Viruses. 2021 May 12;13(5):895. doi: 10.3390/v13050895.
The aim of this study was to determine whether self-collected pure saliva (SCPS) is comparable to nasopharyngeal (NP) swabs in the quantitative detection of SARS-CoV-2 by RT-PCR in asymptomatic, mild patients with confirmed COVID-19. Thirty-one patients aged from 18 to 85 years were included between 9 June and 11 December 2020. A SCPS sample and a NP sample were taken for each patient. Quantitative PCR was performed to detect SARS-CoV-2 viral load. Results of SCPS vs. NP samples testing were compared. Statistical analyses were performed. Viral load was significantly correlated ( = 0.72). The concordance probability was estimated at 73.3%. In symptomatic adults, SCPS performance was similar to that of NP swabs (Percent Agreement = 74.1%; = 0.11). Thus, the salivary test based on pure oral saliva samples easily obtained by noninvasive techniques has a fair agreement with the nasopharyngeal one in asymptomatic, mild patients with a confirmed diagnosis of COVID-19.
本研究旨在确定在确诊 COVID-19 的无症状、轻症患者中，与鼻咽拭子（NP）相比，自我采集的纯唾液（SCPS）是否可用于 RT-PCR 定量检测 SARS-CoV-2。2020 年 6 月 9 日至 12 月 11 日期间，共纳入 31 名年龄在 18 至 85 岁的患者。每位患者均采集了 SCPS 样本和 NP 样本。采用定量 PCR 法检测 SARS-CoV-2 病毒载量。比较了 SCPS 样本与 NP 样本的检测结果。进行了统计学分析。病毒载量呈显著相关（ = 0.72）。一致性概率估计为 73.3%。在有症状的成年人中，SCPS 的表现与 NP 拭子相似（一致性百分比=74.1%； = 0.11）。因此，基于纯口腔唾液样本的唾液检测与确诊 COVID-19 的无症状、轻症患者的鼻咽拭子具有良好的一致性。
