Fishery Machinery and Instrument Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200092, China.
East China Normal University, Shanghai 200241, China.
Int J Environ Res Public Health. 2021 May 6;18(9):4941. doi: 10.3390/ijerph18094941.
Anaerobic ammonium oxidation (anammox) is a key biochemical process to reduce nitrogen pollution in aquaculture, especially in water recirculating pond aquaculture system (RPAS). We used 16S RNA and quantified PCR to study the distribution and environmental impacts of anammox bacteria in RPAS. The results show that the anammox bacterial community distributions and diversities that are apparently unit-specific and seasonal have significant ( < 0.05) difference variation in the RPAS. Most of the anaerobic ammonium oxidation bacteria sequences (77.72%) retrieved from the RPAS belong to the Brocadia cluster. The abundance of anammox bacterial in the RPAS ranged from 3.33 × 101 to 41.84 × 101 copies per ng of DNA. The environmental parameter of temperature and nitrogen composition in water could have impacted the anammox bacterial abundance. This study provides more information on our understanding of the anammox bacteria in the RPAS, and provides an important basis for RPAS improvement and regulation.
厌氧氨氧化（anammox）是减少水产养殖氮污染的关键生化过程，特别是在水再循环池塘水产养殖系统（RPAS）中。我们使用 16S RNA 和定量 PCR 来研究 RPAS 中厌氧氨氧化菌的分布和环境影响。结果表明，RPAS 中厌氧氨氧化菌群落的分布和多样性具有明显的特定单元和季节性差异（<0.05）。从 RPAS 中获得的大多数厌氧氨氧化细菌序列（77.72%）属于 Brocadia 群。RPAS 中厌氧氨氧化菌的丰度范围为 3.33×101 到 41.84×101 拷贝每 ng DNA。水温和氮组成等环境参数可能会影响厌氧氨氧化菌的丰度。本研究为我们理解 RPAS 中的厌氧氨氧化菌提供了更多信息，并为 RPAS 的改进和调控提供了重要依据。
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