Institute for Global Change Adaptation Science, Ibaraki University, Mito, Ibaraki 310-8512, Japan.
Microbes Environ. 2012;27(3):316-9. doi: 10.1264/jsme2.me11330. Epub 2012 Feb 22.
An anammox assay involving a ¹⁵N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N₂ production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca. 'B. anammoxidans', and Ca. 'Kuenenia stuttgartiensis'. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.
采用¹⁵N 示踪剂和气相色谱-质谱联用的厌氧氨氧化测定法显示，日本沟壑稻田中，潜在的厌氧氨氧化活性占总 N₂生成量的 1%至 5%。在四个 4 厘米深的土层中，顶层的活性最高。克隆文库显示，顶层的 DNA 含有与“布鲁卡氏菌属富养菌”、“布鲁卡氏菌属厌氧氨氧化菌”和“斯图尔特氏菌属卡恩氏菌”相关的序列。这些结果表明，尽管一小部分氮气气体通过厌氧氨氧化从土壤中排放出来，但稻田土壤中确实存在特定的厌氧氨氧化细菌种群。
