通过生物催化生产醛类

Kazimírová Veronika, Rebroš Martin

Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, 811 07 Bratislava, Slovakia.

Int J Mol Sci. 2021 May 6;22(9):4949. doi: 10.3390/ijms22094949.

The production of aldehydes, highly reactive and toxic chemicals, brings specific challenges to biocatalytic processes. Absence of natural accumulation of aldehydes in microorganisms has led to a combination of in vitro and in vivo strategies for both, bulk and fine production. Advances in genetic and metabolic engineering and implementation of computational techniques led to the production of various enzymes with special requirements. Cofactor synthesis, post-translational modifications and structure engineering are applied to prepare active enzymes for one-step or cascade reactions. This review presents the highlights in biocatalytical production of aldehydes with the potential to shape future industrial applications.

醛类作为高反应性和有毒化学品，其生产给生物催化过程带来了特殊挑战。微生物中不存在醛类的天然积累，这导致了用于大规模和精细生产的体外和体内策略的结合。基因和代谢工程的进展以及计算技术的应用，催生了各种具有特殊要求的酶。辅因子合成、翻译后修饰和结构工程被应用于制备用于一步或级联反应的活性酶。本文综述了生物催化生产醛类的研究亮点，这些亮点有可能塑造未来的工业应用。