Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Strasse 4, 17487, Greifswald, Germany.
Novartis Institutes for BioMedical Research, Global Discovery Chemistry, 4056, Basel, Switzerland.
Angew Chem Int Ed Engl. 2021 Jan 4;60(1):88-119. doi: 10.1002/anie.202006648. Epub 2020 Aug 17.
Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes in organic synthesis, especially to make chiral compounds for pharmaceuticals as well for the flavors and fragrance industry, are the most prominent examples. In addition, biocatalysts are used on a large scale to make specialty and even bulk chemicals. This review intends to give illustrative examples in this field with a special focus on scalable chemical production using enzymes. It also discusses the opportunities and limitations of enzymatic syntheses using distinct examples and provides an outlook on emerging enzyme classes.
生物催化作为化学催化的替代方法，在各个领域得到了广泛的应用。酶在有机合成中的应用，特别是用于制药以及香精香料行业的手性化合物的合成，是最突出的例子。此外，生物催化剂还被大规模用于生产特种甚至大宗化学品。本文综述了该领域的实例，特别关注使用酶进行可扩展的化学生产。本文还通过不同的实例讨论了酶合成的机会和限制，并对新兴的酶类进行了展望。
