Lin Xuejun, Li Ruimei, Zhou Yangjiao, Tang Fenlian, Wang Yajie, Lu Xiaohua, Wang Shijia, Yao Yuan, Liu Jiao, Hu Xinwen, Guo Jianchun
College of Tropical Crops, Hainan University, Haikou 570228, China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Plants (Basel). 2021 May 8;10(5):941. doi: 10.3390/plants10050941.
Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to was isolated from the cassava genome and named . It contains domains specific to annexins, including four annexin repeat sequences (I-IV), a Ca-binding sequence, Ca-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of reduced the sensitivity of transgenic to various Ca, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene and auxin signaling pathway-related genes and in transgenic was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the gene may increase the salt tolerance of transgenic via the IAA signaling pathway.
膜联蛋白是一类可溶性的钙依赖性磷脂结合蛋白超家族，在植物中具有相当大的调节作用，尤其是在应对逆境和胁迫时。据报道，该基因在各种非生物胁迫反应中发挥重要作用。在我们的研究中，从木薯基因组中分离出一个与[具体基因名称]高度相似的膜联蛋白基因的cDNA，并将其命名为[具体基因名称]。它包含膜联蛋白特有的结构域，包括四个膜联蛋白重复序列（I-IV）、一个钙结合序列、与钙无关的膜结合相关色氨酸残基以及一个盐桥相关结构域。[具体基因名称]定位于细胞膜和细胞质中，并且发现它在木薯的贮藏根中优先表达。[具体基因名称]的过表达降低了转基因[具体植物名称]对各种钙、氯化钠和吲哚-3-乙酸（IAA）浓度的敏感性。在盐胁迫下，转基因[具体植物名称]中与抗逆相关基因[具体基因名称]以及生长素信号通路相关基因[具体基因名称]和[具体基因名称]的表达显著增加，而丙二醛（MDA）含量显著低于对照。这些结果表明，[具体基因名称]基因可能通过IAA信号通路提高转基因[具体植物名称]的耐盐性。
