Wang Ya-Ping, Liu Lin, Wang Xue-Shan, Hong Kun-Qiang, Zhang Li-Hua, Sun Zhong-Guan, Xiao Dong-Guang
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Life Science, Zaozhuang University, Zaozhuang 277160, China.
Bioengineering (Basel). 2021 May 8;8(5):61. doi: 10.3390/bioengineering8050061.
Uncoordinated carbon-nitrogen ratio in raw materials will lead to excessive contents of higher alcohols in alcoholic beverages. The effect of gene, the GATA transcription activator, on higher alcohol biosynthesis was investigated to clarify the mechanism of regulating higher alcohol metabolism under high concentrations of free amino nitrogen (FAN). The availability of FAN by strain SDT1K with a double-copy deletion was 28.31% lower than that of parent strain S17, and the yield of higher alcohols was 33.91% lower. The transcript levels of the downstream target genes of and higher alcohol production in the double-copy deletion mutant suggested that a part of the effect of deletion on higher alcohol production was the downregulation of , , and . This study shows that GATA factors can effectively regulate the metabolism of higher alcohols in and provides valuable insights into higher alcohol biosynthesis, showing great significance for the wheat beer industry.
原料中不协调的碳氮比会导致酒精饮料中高级醇含量过高。研究了GATA转录激活因子基因对高级醇生物合成的影响，以阐明在高浓度游离氨基氮（FAN）条件下调节高级醇代谢的机制。双拷贝缺失菌株SDT1K的FAN利用率比亲本菌株S17低28.31%，高级醇产量低33.91%。双拷贝缺失突变体中该基因下游靶基因的转录水平和高级醇产量表明，该基因缺失对高级醇产量的部分影响是通过下调该基因、该基因和该基因实现的。本研究表明，GATA因子可以有效调节该物质中高级醇的代谢，为高级醇生物合成提供了有价值的见解，对小麦啤酒行业具有重要意义。
