YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells.

encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the and gene, respectively. In contrast to the well-established roles of the latter two in gene regulation, X chromosome inactivation and binding to specific transposable elements (TEs), much less is known about YY2, and its presence during mouse preimplantation development has not been described. As it has been reported that mouse embryonic stem cells (mESC) cannot be propagated in the absence of , the mechanistic understanding of how contributes to mESC maintenance remains only very partially characterized. We describe expression studies using RT-PCR and staining with a high-affinity polyclonal serum in mouse embryos and mESC. Although YY2 is expressed during preimplantation development, its presence appears dispensable for developmental progress in vitro until formation of the blastocyst. Attenuation of levels failed to alter either levels in two-cell embryos or IAP and MERVL levels at later preimplantation stages. In contrast to previous claims that constitutively expressed shRNA against in mESC prohibited the propagation of mESC in culture, we obtained colonies generated from mESC with attenuated levels. Concomitant with a decreased number of undifferentiated colonies, -depleted mESC expressed higher levels of but no differences in the expression of TEs or other pluripotency markers including , , and were observed. These results confirm the contribution of to the maintenance of mouse embryonic stem cells and show the preimplantation expression of YY2. These functions are discussed in relation to mammalian-specific functions of YY1 and REX1.