Kamolsukyeunyong Wintai, Sukhaket Wissarut, Pitija Kitsada, Thorngkham Pornwalai, Mahatheeranont Sugunya, Toojinda Theerayut, Vanavichit Apichart
National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, Thailand.
Expert Center of Innovative Agriculture, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Technopolis, Khlong Ha, Khlong Luang, Pathum Thani 12120, Thailand.
Plants (Basel). 2021 May 22;10(6):1049. doi: 10.3390/plants10061049.
The rice sesquiterpene synthase II gene (, LOC_Os04g27430), which is involved in the antixenosis defense mechanism of rice against brown planthopper (BPH) infestation, was identified in the BPH-resistant rice variety Rathu Heenati (RH). In contrast, the gene was not functional in the BPH-susceptible rice variety KDML105 (KD). Single-nucleotide polymorphisms (SNPs) in the promoter region and in exon 5 of the gene and a seven amino acid deletion in the deduced protein sequence are suggested as factors that negatively regulate the function of the gene. Sequence analysis of the promoter region and expression analysis of the gene in several rice genotypes revealed the correlation of SNPs of the ATHB-1, SBE1, and P-factor with the expression of the gene. Genomic and complementary DNA (cDNA) sequence analysis at exon 5 of the gene showed that the 21 bp deletion naturally occurred in several rice genotypes. The antixenosis of the BPH feeding preference (AFP) of rice varieties differed in the seven amino acid deletion lesion of the gene, suggesting that the seven amino acid deletion negatively controls the antixenosis mechanism during BPH infestation. Analysis of the plant volatile compounds released after BPH infestation suggested that E-β-farnesene (EBF) is the major product of the gene.
水稻倍半萜合酶II基因（LOC_Os04g27430）参与水稻对褐飞虱（BPH）侵害的抗生性防御机制，该基因在抗BPH水稻品种拉杜希纳蒂（RH）中被鉴定出来。相比之下，该基因在感BPH水稻品种KDML105（KD）中无功能。该基因启动子区域和第5外显子中的单核苷酸多态性（SNP）以及推导蛋白序列中的7个氨基酸缺失被认为是对该基因功能产生负调控的因素。对启动子区域的序列分析以及该基因在几种水稻基因型中的表达分析揭示了ATHB - 1、SBE1和P因子的SNP与该基因表达的相关性。对该基因第5外显子的基因组和互补DNA（cDNA）序列分析表明，21 bp缺失在几种水稻基因型中自然发生。水稻品种对BPH取食偏好的抗生性（AFP）在该基因的7个氨基酸缺失损伤方面存在差异，这表明7个氨基酸缺失在BPH侵害期间对抗生性机制产生负调控。对BPH侵害后释放的植物挥发性化合物的分析表明，E-β-法尼烯（EBF）是该基因的主要产物。
