Archer L M, Dusanic D G
Department of Life Sciences, Indiana State University, Terre Haute 47809.
Southeast Asian J Trop Med Public Health. 1988 Mar;19(1):95-100.
Vero cells, SP2/O-Ag 14 myeloma cells and 4B87 hybridoma cells were stored either at refrigeration (5 degrees C) or freezing (-18 degrees C) temperatures. Cells were recovered every five days and percentages of viable cells were determined by the trypan blue exclusion staining method before the cells were incubated at 37 degrees C in a 5% CO2 atmosphere. SP2/0 cells grew after 30 days of storage at 5 degrees C. Hybridoma (4B87) cells survived 20 days of cold storage in HY medium and maintained antibody production. For each cell type, higher percentages of viable cells were observed among cells stored in HY medium than among cells stored in DMEM. Vero cells stored for 40 days at 5 degrees C grew when removed to optimal conditions of 37 degrees C and 5% CO2. There was no growth of cells recovered after storage at -18 degrees C.
Vero细胞、SP2/O-Ag 14骨髓瘤细胞和4B87杂交瘤细胞分别保存在冷藏（5摄氏度）或冷冻（-18摄氏度）温度下。每五天复苏一次细胞，并在将细胞于37摄氏度、5%二氧化碳气氛中孵育之前，通过台盼蓝排斥染色法测定活细胞百分比。SP2/0细胞在5摄氏度下储存30天后生长。杂交瘤（4B87）细胞在HY培养基中冷藏20天后存活，并维持抗体产生。对于每种细胞类型，在HY培养基中储存的细胞中观察到的活细胞百分比高于在DMEM中储存的细胞。在5摄氏度下储存40天的Vero细胞转移到37摄氏度和5%二氧化碳的最佳条件下时生长。在-18摄氏度储存后复苏的细胞没有生长。
