Pĕknicová J, Landa V
Folia Biol (Praha). 1985;31(5):340-7.
Hybridoma technology requires freezing of parental myeloma cells, continuous freezing and thawing of clones and stable hybridoma cells. A modification of the method of two-step freezing is described. The cryoprotective agent (5% dimethyl sulphoxide) is added to the cells at room temperature for 10 min. Cells are then transferred directly to the -25 degrees C bath, held at this temperature for 10 min, and stored directly in liquid nitrogen. Thawing is rapid in a water bath warmed to 60-80 degrees C. Hybridoma cells retain high viability and the production of specific monoclonal antibody after thawing.
杂交瘤技术需要冷冻亲本骨髓瘤细胞，对克隆和稳定的杂交瘤细胞进行连续冻融。本文描述了两步冷冻方法的一种改进。在室温下将冷冻保护剂（5%二甲基亚砜）加入细胞中10分钟。然后将细胞直接转移到-25℃的浴中，在此温度下保持10分钟，然后直接储存在液氮中。在加热到60-80℃的水浴中快速解冻。解冻后杂交瘤细胞保持高活力并产生特异性单克隆抗体。
