Plant Sciences Unit, Flanders Research Institute for Agricultural, Fisheries and Food Research (ILVO), Caritasstraat 39, 9090 Melle, Belgium.
Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Genes (Basel). 2021 May 13;12(5):730. doi: 10.3390/genes12050730.
Breeding programs in ornamentals can be facilitated by integrating knowledge of phylogenetic relatedness of potential parents along with other genomic information. Using AFLP, genetic distances were determined for 59 genotypes, comprising 55 commercial cultivars of the three subgenera of a total collection of 61 genotypes. A subgroup of 45 genotypes, including intragroup and intergroup hybrids, were selected and further characterized for genome sizes and chromosome numbers. The variation in genome size ranged from 1.51 ± 0.01 pg/2C to 12.94 ± 0.07 pg/2C. The chromosome numbers ranged from 26 to 108-110 with some hybrids showing an aberrant number of chromosomes based on their parents' constitution. All chromosome numbers of are an even number, which presumes that unreduced gametes occur in some cross combinations. Overall, parental difference in genome size and chromosome number were not limiting for cross compatibility. Good crossing compatibility was correlated to a Jaccard similarity coefficient as parameter for parental relatedness of about 0.5. Additionally, parent combinations with high differences in the DNA/chromosome value could not result in a successful cross. We expect that our results will enable breeding programs to overcome crossing barriers and support further breeding initiatives.
通过整合潜在亲本系统发育关系的知识以及其他基因组信息，可以促进观赏植物的繁殖计划。使用 AFLP，对 59 个基因型，包括三个亚属的 55 个商业品种，总共 61 个基因型的遗传距离进行了测定。选择了包括组内和组间杂种在内的 45 个基因型亚组，进一步对其基因组大小和染色体数目进行了特征描述。基因组大小的变化范围为 1.51 ± 0.01 pg/2C 至 12.94 ± 0.07 pg/2C。染色体数目从 26 到 108-110 不等，一些杂种根据其亲本的组成显示出异常数量的染色体。的所有染色体数目都是偶数，这表明在某些杂交组合中会发生未减数的配子。总体而言，基因组大小和染色体数目的亲本差异并不是杂交相容性的限制因素。良好的杂交相容性与亲本亲缘关系的杰卡德相似系数相关，约为 0.5。此外，DNA/染色体值差异较大的亲本组合不能导致成功的杂交。我们预计，我们的结果将使繁殖计划能够克服杂交障碍，并支持进一步的繁殖计划。
