Kirov Ilya, Khrustaleva Ludmila, Laere Katrijn Van, Soloviev Alexander, Romanov Dmitry, Fesenko Igor
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Laboratory of proteomics, Miklukho-Maklaya, 16/10,117997, Moscow, Russia.
Russian State Agrarian University-MTAA, Timiryazevskay str. 49, 127550 Moscow, Russia.
Comp Cytogenet. 2017 Nov 21;11(4):747-757. doi: 10.3897/CompCytogen.v11i4.20830. eCollection 2017.
An idiogram construction following chromosome measurements is a versatile tool for cytological, cytogenetic and phylogenetic studies. The information on chromosome length, centromere index and position of cytogenetic landmarks along with modern techniques (e.g. genomic and fluorescence in situ hybridization, banding, chromosome painting) can help to shed light on genome constitution, chromosome rearrangements and evolution. While idiogram construction is a routine task there are only few freely available programs that can perform chromosome measurements and no software for simultaneous measuring of chromosome parameters, chromosomal landmark and FISH signal positions and idiogram construction. To fill this gap, we developed DRAWID (DRAWing IDiogram), java-based cross-platforming program for chromosome analysis and idiogram construction. DRAWID has number of advantages including a user-friendly interactive interface, possibility for simultaneous chromosome and FISH/GISH/banding signal measurement and idiogram drawing as well as number of useful functions facilitating the procedure of chromosome analysis. The output of the program is Microsoft XL table and publish-ready idiogram picture. DRAWID and the manual for its use are freely available on the website at: http://www.drawid.xyz.
根据染色体测量构建核型模式图是一种用于细胞学、细胞遗传学和系统发育研究的多功能工具。染色体长度、着丝粒指数以及细胞遗传学标记位置等信息，再结合现代技术（如基因组和荧光原位杂交、显带、染色体涂染），有助于揭示基因组构成、染色体重排和进化情况。虽然构建核型模式图是一项常规任务，但仅有少数免费程序可进行染色体测量，且没有能同时测量染色体参数、染色体标记及荧光原位杂交信号位置并构建核型模式图的软件。为填补这一空白，我们开发了DRAWID（绘制核型模式图），这是一个基于Java的跨平台程序，用于染色体分析和核型模式图构建。DRAWID具有诸多优点，包括用户友好的交互式界面、可同时测量染色体及荧光原位杂交/基因组原位杂交/显带信号并绘制核型模式图，以及许多有助于染色体分析过程的实用功能。该程序的输出结果是Microsoft XL表格和可供发表的核型模式图图片。DRAWID及其使用手册可在以下网站免费获取：http://www.drawid.xyz 。
