一种用于动物新冠病毒病血清学诊断的灵敏且特异的竞争性酶联免疫吸附测定法。
A Sensitive and Specific Competitive Enzyme-Linked Immunosorbent Assay for Serodiagnosis of COVID-19 in Animals.
作者信息
Lau Susanna K P, He Zirong, Tsang Chi-Ching, Chan Tony T Y, Luk Hayes K H, Chan Elaine, Li Kenneth S M, Fung Joshua, Chow Franklin W N, Tam Anthony R, Chung Tom W H, Wong Sally C Y, Que Tak-Lun, Fung Kitty S C, Lung David C, Wu Alan K L, Hung Ivan F N, Teng Jade L L, Wernery Ulrich, Hui Suk-Wai, Martelli Paolo, Woo Patrick C Y
机构信息
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Department of Medicine, Queen Mary Hospital, Pokfulam, Hong Kong.
出版信息
Microorganisms. 2021 May 10;9(5):1019. doi: 10.3390/microorganisms9051019.
In addition to human cases, cases of COVID-19 in captive animals and pets are increasingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inactive SARS-CoV-2 virions, COVID-19-negative animals, and animals seropositive to other betacoronaviruses. A cut-off percent inhibition value of 22.345% was determined and the analytical sensitivity and specificity were found to be 1:64-1:256 and 93.9%, respectively. Evaluation on its diagnostic performance using 155 serum samples from COVID-19-negative animals and COVID-19 human patients showed a diagnostic sensitivity and specificity of 80.8% and 100%, respectively. The cELISA can be incorporated into routine blood testing of farmed/captive animals for COVID-19 surveillance.
除人类病例外，圈养动物和宠物感染新冠病毒的病例报告也日益增多。这引发了对新冠病毒在人和动物之间双向传播的担忧。在此，我们开发了一种基于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核衣壳蛋白的竞争酶联免疫吸附测定法（cELISA）用于新冠病毒的血清学诊断，理论上可用于几乎所有种类的动物。我们使用了187份来自感染/未感染新冠病毒患者的血清样本、用灭活的SARS-CoV-2病毒粒子免疫的实验动物、新冠病毒阴性动物以及对其他β冠状病毒血清学阳性的动物。确定了22.345%的抑制率截断值，分析灵敏度和特异性分别为1:64至1:256和93.9%。使用来自新冠病毒阴性动物和新冠病毒患者的155份血清样本对其诊断性能进行评估，结果显示诊断灵敏度和特异性分别为80.8%和100%。该cELISA可纳入养殖/圈养动物的常规血液检测中，用于新冠病毒监测。
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