Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland.
Laboratory of Structural Biochemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza, 80-308 Gdansk, Poland.
Int J Environ Res Public Health. 2021 May 10;18(9):5041. doi: 10.3390/ijerph18095041.
Pectinolytic bacteria from the genus cause high economic losses in various crops, vegetables, and ornamentals including potato. Thus far, these strains have been isolated from distinct environments such as rotten or asymptomatic plants, soil, and waterways. The prevalence of soft rot in different depths of Pomeranian lakes was performed by a qualified scuba diver over 2 years of monitoring. It allowed for the isolation and broad characterization of a strain from the newly established species . Phylogenetic analysis on the sequences of and genes revealed the highest similarity of this strain to CFBP 8637. In addition to the determination of analytical profile index (API 20E), we discovered that this strain possesses a smooth form of a lipopolysaccharide with O-polysaccharide consisting of mannose, glucose, and abequose. Moreover, the characterized strain, described as IFB5637, produced plant-cell-wall-degrading enzymes, such as pectinases, cellulases, proteases, and was capable of macerating potato and chicory tissues under laboratory conditions. In view of more frequent irrigation of seed potato fields resulting from the ongoing climate warming, it is important to monitor the occurrence of potential disease-causing agents in natural waterways.
果胶分解菌属中的细菌会导致包括土豆在内的各种作物、蔬菜和观赏植物遭受高经济损失。迄今为止，这些菌株已从不同的环境中分离出来，如腐烂或无症状的植物、土壤和水道。在 2 年多的监测过程中，一名合格的水肺潜水员在不同深度的波美拉尼亚湖进行了软腐病的流行情况调查。这使得从新建立的物种中分离和广泛表征出一株菌成为可能。基于 和 基因序列的系统发育分析表明，该菌株与 CFBP 8637 的相似度最高。除了确定分析概况指数（API 20E）之外，我们还发现该菌株具有光滑形式的脂多糖，其 O-多糖由甘露糖、葡萄糖和艾杜糖组成。此外，该菌株被描述为 IFB5637，可产生植物细胞壁降解酶，如果胶酶、纤维素酶、蛋白酶，并能够在实验室条件下使土豆和菊苣组织软化。鉴于气候变暖导致种薯田灌溉更加频繁，监测天然水道中潜在致病因子的发生情况非常重要。
