Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea.
Genes (Basel). 2021 May 14;12(5):745. doi: 10.3390/genes12050745.
Shoot branching is considered as an important trait for the architecture of plants and contributes to their growth and productivity. In cereal crops, such as rice, shoot branching is controlled by many factors, including phytohormones signaling networks, operating either in synergy or antagonizing each other. In rice, shoot branching indicates the ability to produce more tillers that are essential for achieving high productivity and yield potential. In the present study, we evaluated the growth and development, and yield components of a doubled haploid population derived from a cross between 93-11 (P1, ) and Milyang352 (P2, ), grown under normal nitrogen and low nitrogen cultivation open field conditions. The results of the phenotypic evaluation indicated that parental lines 93-11 (P1, a high tillering cultivar) and Milyang352 (P2, a low tillering cultivar) showed distinctive phenotypic responses, also reflected in their derived population. In addition, the linkage mapping and quantitative trait locus (QTL) analysis detected three QTLs associated with tiller number on chromosome 2 (, 130 cM, logarithm of the odds (LOD) 4.14, PVE 14.5%; and , 134 cM, LOD: 6.05, PVE: 20.5%) and chromosome 4 (, 134 cM, LOD 3.92, PVE 14.5%), with having the highest phenotypic variation explained, and the only QTL associated with tiller number under low nitrogen cultivation conditions, using Kompetitive Allele-Specific PCR (KASP) and Fluidigm markers. The additive effect (1.81) of indicates that the allele from 93-11 (P1) contributed to the observed phenotypic variation for tiller number under low nitrogen cultivation. The breakthrough is that the majority of the candidate genes harbored by the QTLs and (here associated with the control of shoot branching under low and normal nitrogen cultivation, respectively), were also proposed to be involved in plant stress signaling or response mechanisms, with regard to their annotations and previous reports. Therefore, put together, these results would suggest that a possible crosstalk exists between the control of plant growth and development and the stress response in rice.
分蘖是植物结构的一个重要特征，有助于其生长和生产力。在谷类作物中，如水稻，分蘖受许多因素的控制，包括植物激素信号网络，它们协同或拮抗作用。在水稻中，分蘖表示产生更多分蘖的能力，这对于实现高生产力和产量潜力至关重要。在本研究中，我们评估了来自 93-11（P1，高分蘖品种）和 Milyang352（P2，低分蘖品种）杂交衍生的双单倍体群体在正常氮和低氮大田条件下的生长发育和产量构成。表型评价的结果表明，亲本系 93-11（P1，高分蘖品种）和 Milyang352（P2，低分蘖品种）表现出明显的表型响应，这也反映在它们的衍生群体中。此外，连锁图谱和数量性状位点（QTL）分析在第 2 号染色体（，130cM，对数优势（LOD）4.14，PVE 14.5%；和，134cM，LOD：6.05，PVE：20.5%）和第 4 号染色体（，134cM，LOD 3.92，PVE 14.5%）上检测到与分蘖数相关的三个 QTL，其中具有最高表型变异解释率，并且是唯一一个与低氮栽培条件下分蘖数相关的 QTL，使用 Kompetitive Allele-Specific PCR（KASP）和 Fluidigm 标记。的加性效应（1.81）表明，来自 93-11（P1）的等位基因有助于在低氮栽培条件下观察到的分蘖数的表型变异。突破点在于，QTL 和 （分别与低氮和正常氮栽培下分蘖的控制有关）所携带的大多数候选基因也被提出参与植物胁迫信号或响应机制，这是基于它们的注释和以前的报道。因此，综上所述，这些结果表明，水稻中植物生长发育的控制与胁迫响应之间可能存在一种串扰。
