Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea.
Department of Southern Area Crop Science, National Institute of Crop Science, RDA, Miryang 50424, Korea.
Genes (Basel). 2021 Feb 20;12(2):298. doi: 10.3390/genes12020298.
A large number of hormonal biosynthetic or signaling pathways genes controlling shoot branching are widely known for their roles in regulating plant growth and development, operating in synergetic or antagonistic manner. However, their involvement in abiotic stress response mechanism remains unexplored. Initially, we performed an in silico analysis to identify potential transcription binding sites for the basic leucine zipper 62 transcription factor (bZIP62 TF) in the target branching related genes. The results revealed the presence of regulatory elements specific to two bZIP TFs, and , rather than . Interestingly, these bZIP TFs were previously proposed to be negatively regulated by the TF under salinity in . Therefore, we investigated the transcriptional regulation of more axillary branching (MAX, strigolactone), PIN-FORMED (PINs, auxin carriers), gibberellic acid (GA)-biosynthetic genes as well as isopentenyltransferase (IPT, cytokinin biosynthesis pathway) genes in response to drought stress in Col-0 wild type. In addition, in the perspective of exploring the transcriptional interplay of the selected genes with the , we measured their expression by qPCR in the (lacking the gene) background under the same conditions. Our findings revealed that the expression of , , and was differentially regulated by drought stress between the and Col-0 wild type, but not Similarly, the transcripts accumulation of and (known as auxin efflux carriers), and that of the showed similar regulation patterns in . However, expression was downregulated in Col-0, but no change was observed in . Furthermore, and exhibited a differential transcripts accumulation pattern in and Col-0 wild type (WT). In the same way, the expression of the GA biosynthetic genes and and that of were differentially regulated in compared to the Col-0. Meanwhile, showed a similar expression pattern with Col-0. Therefore, all results suggest PIN, MAX, IPT, and GA-biosynthetic genes, which are differentially regulated by transcription factor, as emerging candidate genes that could be involved in drought stress response mechanism in .
大量控制分枝的激素生物合成或信号通路基因，因其在调节植物生长和发育中的作用而广为人知，它们以协同或拮抗的方式发挥作用。然而，它们在非生物胁迫反应机制中的参与尚未得到探索。最初，我们进行了计算机分析，以鉴定靶分枝相关基因中基本亮氨酸拉链 62 转录因子（bZIP62 TF）的潜在转录结合位点。结果表明，存在两种 bZIP TF 的特定调节元件，而不是 。有趣的是，这些 bZIP TF 先前被提议在盐胁迫下受到 TF 的负调控。因此，我们研究了更多腋生分枝（MAX，Strigolactone）、PIN 形成（PINs，生长素载体）、赤霉素（GA）-生物合成基因以及异戊烯基转移酶（IPT，细胞分裂素生物合成途径）基因在 Col-0 野生型响应干旱胁迫时的转录调控。此外，为了探索所选基因与 之间的转录相互作用，我们在相同条件下在 （缺乏 基因）背景下通过 qPCR 测量了它们的表达。我们的研究结果表明，干旱胁迫下， 、 、和 的表达在 Col-0 野生型和 之间存在差异调节，但 则不同。同样， （已知是生长素外排载体）和 的转录物积累以及 的表达在 中表现出相似的调控模式。然而，在 Col-0 中下调了 的表达，但 在 中没有观察到变化。此外，在 中， 和 表现出与 Col-0 野生型不同的转录物积累模式。同样，GA 生物合成基因 和 的表达以及 的表达在 中与 Col-0 存在差异调节。同时， 在 Col-0 中表现出相似的表达模式。因此，所有结果表明，受 转录因子差异调控的 PIN、MAX、IPT 和 GA 生物合成基因可能是参与 干旱胁迫反应机制的候选基因。
