Sosa-Gutierrez Carolina Guadalupe, Cervantes-Castillo Maria Almudena, Laguna-Gonzalez Ramon, Lopez-Echeverria Laura Yareli, Ojeda-Ramírez Deyanira, Oyervides Mayra
Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo, Hidalgo 43600, Mexico.
BioGeneticks and Other Vector Diseases Lab., Tulancingo, Hidalgo 43660, Mexico.
Diseases. 2021 May 14;9(2):37. doi: 10.3390/diseases9020037.
Human granulocytic Anaplasmosis (HGA), is a tick-borne infectious disease transmitted by ticks, resulting in acute feverish episodes. The etiological agent is the bacteria ; which is spread by ticks of the genus . to complete its life cycle. In Mexico, there is only one case report. The primary challenge is understanding how other bacteria affect or overlap with the clinical manifestation of the disease. Sample collection occurred over the period September 2017 through October 2019. Blood samples from human subjects were obtained immediately after they signed consent forms. We analyzed for the presence for by serological (IFA IgG two times) and PCR targeting and genes, followed by DNA sequencing. All patients with a history of travel abroad were dismissed for this project. In total, 1924 patients participated and of these, 1014 samples across the country were analyzed. Of these, 85 (8.38%) had IFA results that ranged from 1:384 to 1:896. Of the positive samples, 7.10% were used for PCR. Significant clinical manifestations included: dizziness, nausea, petechial, epistaxis, enlarged liver and/or spleen and thrombocytopenia. Hospitalization of at least 1.5 days was necessary for 3.2% of patients. None of the cases analyzed were lethal. This is the first clinical manifestations along with serological test results and molecular analysis confirmed the presence of resulting in HGA in patients from Mexico. Health institutions and medical practitioners in general should include diagnostic testing for HGA among high risk populations and should recognize it as a vector-borne emerging infectious disease in Mexico.
人粒细胞无形体病（HGA）是一种由蜱传播的蜱媒传染病，可导致急性发热发作。病原体是细菌，由 属的蜱传播以完成其生命周期。在墨西哥，仅有一例病例报告。主要挑战在于了解其他细菌如何影响该疾病的临床表现或与之重叠。样本采集于2017年9月至2019年10月期间进行。在人类受试者签署同意书后立即采集血样。我们通过血清学方法（两次间接免疫荧光法检测IgG）以及针对 基因和 基因的聚合酶链反应（PCR）分析 的存在情况，随后进行DNA测序。所有有出国旅行史的患者均被排除在本项目之外。共有1924名患者参与，其中对全国范围内的1014份样本进行了分析。在这些样本中，85份（8.38%）间接免疫荧光法检测结果在1:384至1:896之间。在阳性样本中，7.10%用于PCR检测。显著的临床表现包括：头晕、恶心、瘀点、鼻出血、肝和/或脾肿大以及血小板减少。3.2%的患者需要住院至少1.5天。所有分析的病例均无致命情况。这是首例关于墨西哥患者中HGA的临床表现，同时血清学检测结果和分子分析证实了 的存在。一般而言，卫生机构和医疗从业者应将HGA的诊断检测纳入高危人群，并应将其视为墨西哥一种新出现的媒介传播传染病。
