Amaral Ana, Fernandes Carina, Szóstek-Mioduchowska Anna, Rebordão Maria Rosa, Skarzynski Dariusz Jan, Ferreira-Dias Graça
CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Departamento de Morfologia e Função, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisboa, Portugal.
Institute of Animal Reproduction and Food Research, Polish Academy of Science, 10-748 Olsztyn, Poland.
Int J Mol Sci. 2021 May 19;22(10):5333. doi: 10.3390/ijms22105333.
Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain () mRNA and COL1 protein relative abundance induced by ELA in endometrial explants of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The mRNA was evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases and treatment times. These findings contribute to the future development of new endometrosis treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare's endometrium and facilitate the therapeutic approach.
子宫内膜异位症是一种导致母马不育的生殖系统病理疾病。我们最近的研究聚焦于中性粒细胞胞外诱捕酶，如弹性蛋白酶（ELA），在马子宫内膜异位症发展过程中的作用。那可丁（NOSC）是一种从罂粟鸦片中提取的生物碱，具有镇咳、抗中风、抗癌和抗纤维化特性。本研究调查了那可丁在体外对卵泡期或黄体中期母马子宫内膜外植体中，经弹性蛋白酶诱导24或48小时后的I型胶原蛋白α2链（）mRNA和COL1蛋白相对丰度的假定抑制作用。通过qPCR评估mRNA，通过蛋白质印迹法评估COL1蛋白相对丰度。在马子宫内膜外植体中，弹性蛋白酶增加了COL1的表达，而那可丁在发情周期各阶段和处理时间均对其有抑制作用。这些发现有助于未来开发新的子宫内膜异位症治疗方法。那可丁可能是一种能够阻止母马子宫内膜胶原蛋白合成并促进治疗的药物。
