Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Sensors (Basel). 2021 May 19;21(10):3545. doi: 10.3390/s21103545.
Choline (Ch) and phosphocholine (PCh) levels in tissues are associated to tissue growth and so to carcinogenesis. Till now, only highly sophisticated and expensive techniques like those based on NMR spectroscopy or GC/LC- high resolution mass spectrometry permitted Ch and PCh analysis but very few of them were capable of a simultaneous determination of these analytes. Thus, a never reported before amperometric biosensor for PCh analysis based on choline oxidase and alkaline phosphatase co-immobilized onto a Pt electrode by co-crosslinking has been developed. Coupling the developed biosensor with a parallel sensor but specific to Ch, a crosstalk-free dual electrode biosensor was also developed, permitting the simultaneous determination of Ch and PCh in flow injection analysis. This novel sensing device performed remarkably in terms of sensitivity, linear range, and limit of detection so to exceed in most cases the more complex analytical instrumentations. Further, electrode modification by overoxidized polypyrrole permitted the development of a fouling- and interferent-free dual electrode biosensor which appeared promising for the simultaneous determination of Ch and PCh in a real sample.
组织中的胆碱（Ch）和磷酸胆碱（PCh）水平与组织生长有关，因此与致癌作用有关。到目前为止，只有基于 NMR 光谱或 GC/LC-高分辨率质谱等高度复杂和昂贵的技术才能分析 Ch 和 PCh，但其中很少有技术能够同时测定这些分析物。因此，我们开发了一种以前从未报道过的基于胆碱氧化酶和碱性磷酸酶共交联固定在 Pt 电极上的电流生物传感器，用于分析 PCh。将开发的生物传感器与平行传感器（但特异性针对 Ch）结合使用，还开发了一种无串扰的双电极生物传感器，可用于在流动注射分析中同时测定 Ch 和 PCh。这种新型传感装置在灵敏度、线性范围和检测限方面表现出色，在大多数情况下超过了更复杂的分析仪器。此外，通过过氧化聚吡咯对电极进行修饰，开发了一种无污垢和干扰的双电极生物传感器，该传感器有望用于同时测定实际样品中的 Ch 和 PCh。
