Ding Jin-Li, Hou Jia, Li Xiu-Hui, Feng Ming-Guang, Ying Sheng-Hua
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
J Fungi (Basel). 2021 May 25;7(6):411. doi: 10.3390/jof7060411.
Mbp1 protein acts as a DNA-binding protein in I cell cycle box-binding complex (MBF) and plays an essential role in filamentous myco-pathogen .In the current study, BbSwi6 (a homologue of yeast Swi6) was functionally characterized in . Both BbSwi6 and BbMbp1 localize in the nucleus and display a direct interaction relationship which is indicated by a yeast two-hybrid assay. significantly contributes to hyphal growth, asexual sporulation and virulence. On the aerial surface, Δ grew slower on various nutrients and displayed abnormal conidia-producing structures, which hardly produced conidia. In liquid media, loss led to 90% reduction in blastospore yield. Finally, the virulence of the Δ mutant was modestly weakened with a reduction of 20% in median lethal time. Comparative transcriptomics revealed that BbSwi6 mediated different transcriptomes during fungal development into conidia and blastospores. Notably, under the indicated condition, the BbSwi6-mediated transcriptome significantly differed to that mediated by BbMbp1. Our results demonstrate that, in addition to their roles as the interactive components in MBF, BbSwi6 and BbMbp1 mediate divergent genetic pathways during morphological transitions in .
Mbp1蛋白在I细胞周期盒结合复合体（MBF）中作为一种DNA结合蛋白发挥作用，并且在丝状真菌病原体中起重要作用。在当前研究中，对BbSwi6（酵母Swi6的同源物）进行了功能表征。BbSwi6和BbMbp1都定位于细胞核，并且酵母双杂交试验表明它们存在直接相互作用关系。BbSwi6对菌丝生长、无性孢子形成和毒力有显著贡献。在气生表面，Δ在各种营养物质上生长较慢，并且分生孢子产生结构异常，几乎不产生分生孢子。在液体培养基中，Δ缺失导致芽生孢子产量降低90%。最后，Δ突变体的毒力略有减弱，中位致死时间减少20%。比较转录组学表明，在真菌发育成分生孢子和芽生孢子的过程中，BbSwi6介导了不同的转录组。值得注意的是，在所示条件下，BbSwi6介导的转录组与BbMbp1介导的转录组有显著差异。我们的结果表明，除了作为MBF中的相互作用成分发挥作用外，BbSwi6和BbMbp1在真菌形态转变过程中介导不同的遗传途径。
