Cong Hao, Li Changgen, Wang Yiming, Zhang Yongjing, Ma Daifu, Li Lianwei, Jiang Jihong
The Key Laboratory of Biotechnology for Medicinal and Edible Plant Resources of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China.
Chinese Academy of Agricultural Sciences Sweet Potato Research Institute, Xuzhou 221131, China.
Microorganisms. 2023 Oct 30;11(11):2666. doi: 10.3390/microorganisms11112666.
() is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi is located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved in cell wall integrity and virulence in several filamentous pathogenic fungi. However, the specific mechanisms by which Swi6 regulates the growth and pathogenicity of plant pathogenic fungi remain elusive. In this study, the deletion mutants and complemented strains of were generated. Deletion of Swi6 in resulted in aberrant growth patterns. Pathogenicity assays on sweet potato storage roots revealed a significant decrease in virulence in the mutant. Non-targeted metabolomic analysis using LC-MS identified a total of 692 potential differentially accumulated metabolites (PDAMs) in the ∆ mutant compared to the wild type, and the results of KEGG enrichment analysis demonstrated significant enrichment of PDAMs within various metabolic pathways, including amino acid metabolism, lipid metabolism, nucleotide metabolism, GPI-anchored protein synthesis, and ABC transporter metabolism. These metabolic pathways were believed to play a crucial role in mediating the growth and pathogenicity of through the regulation of CWI. Firstly, the deletion of the gene led to abnormal amino acid and lipid metabolism, potentially exacerbating energy storage imbalance. Secondly, significant enrichment of metabolites related to GPI-anchored protein biosynthesis implied compromised cell wall integrity. Lastly, disruption of ABC transport protein metabolism may hinder intracellular transmembrane transport. Importantly, this study represents the first investigation into the potential regulatory mechanisms of in plant filamentous pathogenic fungi from a metabolic perspective. The findings provide novel insights into the role of in the growth and virulence of , highlighting its potential as a target for controlling this pathogen.
（）是一种臭名昭著的致病真菌，可引起甘薯黑腐病。真菌中的APSES转录因子Swi6位于细胞壁完整性（CWI）-丝裂原活化蛋白激酶（MAPK）信号通路的下游，并且已被确定在几种丝状致病真菌中参与细胞壁完整性和毒力。然而，Swi6调节植物致病真菌生长和致病性的具体机制仍不清楚。在本研究中，构建了（）的缺失突变体和互补菌株。在（）中缺失Swi6导致生长模式异常。对甘薯贮藏根的致病性测定表明，突变体的毒力显著降低。使用LC-MS的非靶向代谢组学分析确定，与野生型相比，∆突变体中共有692种潜在的差异积累代谢物（PDAM），KEGG富集分析结果表明，PDAM在各种代谢途径中显著富集，包括氨基酸代谢、脂质代谢、核苷酸代谢、GPI锚定蛋白合成和ABC转运蛋白代谢。据信这些代谢途径通过调节CWI在介导（）的生长和致病性中起关键作用。首先，（）基因的缺失导致氨基酸和脂质代谢异常，可能加剧能量储存失衡。其次，与GPI锚定蛋白生物合成相关的代谢物显著富集意味着细胞壁完整性受损。最后，ABC转运蛋白代谢的破坏可能会阻碍细胞内跨膜运输。重要的是，本研究代表了首次从代谢角度对（）在植物丝状致病真菌中的潜在调控机制进行的研究。这些发现为（）在（）的生长和毒力中的作用提供了新的见解，突出了其作为控制这种病原体靶点的潜力。
