School of Veterinary and Animal Science, Federal University of Goiás, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil.
Environmental Virology Laboratory, Water Pollution Research Department, National Research Centre, Dokki, Giza 12622, Egypt.
Genes (Basel). 2021 May 25;12(6):806. doi: 10.3390/genes12060806.
Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro Pé-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against , sp., , leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of was present in 70% of the animals that tested positive for infection. Haplotypes of and and were more common in seronegative animals. Haplotypes related to the gene were associated with animals negative to all infections. Curraleiro Pé-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and infections.
疾病易感性是遗传的，可以在人群之间传播。与免疫反应相关的基因中的单核苷酸多态性（SNP）与牛的疾病有关。本研究调查了 702 份 Curraleiro Pé-Duro 牛细胞因子基因组区域的 SNP，并将其与布鲁氏菌病、钩端螺旋体病、新孢子虫病、白血病、传染性牛鼻气管炎（IBR）和牛病毒性腹泻（BVD）检测中的抗体发生情况相关联。通过竞争性等位基因特异性聚合酶链反应（KASP）方法评估 DNA 样本，以识别多态性。利用 PHASE 2.1.1 软件确定配子相和 SNP 单倍型。使用单变量分析和逻辑回归，将单倍型与针对 、 、 、白血病、传染性鼻气管炎和 BVD 的血清学结果相关联。感染 呈阳性的动物中，有 70%存在 2 号单倍型。 和 以及 单倍型在血清阴性动物中更为常见。与 基因相关的单倍型与所有感染均呈阴性的动物有关。Curraleiro Pé-Duro 牛表现出与细菌、病毒和 感染的抗性相关的多态性。
