Trombitaș Veronica-Elena, Nagy Alina Anda, Berce Cristian, Pall Emoke, Tăbăran Flaviu, Ilea Aranka, Albu Silviu
II-nd Department of Otolaryngology, Iuliu Hațieganu University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania.
Department of Experimental Medicine, Iuliu Hațieganu University of Medicine and Pharmacy, 400000 Cluj-Napoca, Romania.
Microorganisms. 2021 May 30;9(6):1182. doi: 10.3390/microorganisms9061182.
It is acknowledged that the treatment of chronic rhinosinusitis (CRS) represents an important challenge for rhinology and for social and economic life. At present, one of the most common treatments for CRS is represented by local corticosteroids followed by endoscopic sinus surgery (ESS). Starting from the example of the mesenchymal stem cell's (MSC) capacity to migrate and to modulate a real response in the nasal mucosa of an allergic rhinitis mouse model, we try to obtain a response in a CRS mouse model, using MSC derived by adipose tissue. The aim of this study is to demonstrate that the MSC can be used in CRS treatment and could change its priorities. Seventy female mice (6 MSC donor mice) were randomized in two stages of study, 32 Aspergillus fumigatus (Af) exposure mice (20 for histological comparison to 1st control mice and 12 for MSC administration, to CRS/MCS model) and 32 control mice (20 for histological comparison to CRS model and 12 for MSC administration and histological control to MSC model); in the first stage, the (Af) CRS mouse model was targeted, in this section were included 64 ( = 32) mice (treated and control group). In order to assess the inflammation level (histological analysis), the animals were euthanized; in the second stage MSCs (1 × 10/animal) were administered intravenously to a total of 24 ( = 24) mice (12 mice from the exposed group and 12 mice from the second control group). After 12 weeks of Af intranasal instillation, the inflammation parameters evaluated indicated a severe diffuse chronic inflammation, associated with diffuse severe hyperplasia and mature diffuse squamous metaplasia. The MSCs' injection via the ophthalmic vein induced important histopathological changes in the CRS experimental group, starting with the presence of MSCs in all samples and continuing with the important degenerative character of inflammation. MSC administration demonstrated a real improvement of CRS evolution on the CRS mouse model.
人们公认，慢性鼻-鼻窦炎（CRS）的治疗对鼻科学以及社会和经济生活而言都是一项重大挑战。目前，CRS最常见的治疗方法之一是局部使用皮质类固醇，其次是鼻内镜鼻窦手术（ESS）。从间充质干细胞（MSC）在变应性鼻炎小鼠模型的鼻黏膜中迁移并调节实际反应的能力实例出发，我们尝试使用脂肪组织来源的MSC在CRS小鼠模型中获得反应。本研究的目的是证明MSC可用于CRS治疗并可能改变其治疗重点。70只雌性小鼠（6只MSC供体小鼠）被随机分为两个研究阶段，32只烟曲霉（Af）暴露小鼠（20只用于与第一对照组进行组织学比较，12只用于向CRS/MCS模型给予MSC）和32只对照小鼠（20只用于与CRS模型进行组织学比较，12只用于给予MSC并作为MSC模型的组织学对照）；在第一阶段，针对（Af）CRS小鼠模型，该组包括64只（=32只）小鼠（治疗组和对照组）。为了评估炎症水平（组织学分析），对动物实施安乐死；在第二阶段，将MSC（1×10/只动物）静脉注射给总共24只（=24只）小鼠（暴露组12只小鼠和第二对照组12只小鼠）。在鼻内滴注Af 12周后，所评估的炎症参数显示为严重的弥漫性慢性炎症，伴有弥漫性严重增生和成熟的弥漫性鳞状化生。通过眼静脉注射MSC在CRS实验组中引起了重要的组织病理学变化，首先是所有样本中均存在MSC，接着是炎症的重要退行性特征。给予MSC证明了在CRS小鼠模型中CRS病情发展有实际改善。
