Division of Nanotechnology and Functional Materials, Department of Material Sciences and Engineering, Ångström Laboratory, Uppsala University, 751 03 Uppsala, Sweden.
Division of Solid-State Physics, Department of Material Sciences and Engineering, Ångström Laboratory, Uppsala University, 751 03 Uppsala, Sweden.
Biosensors (Basel). 2021 May 28;11(6):173. doi: 10.3390/bios11060173.
This work explores several issues of importance for the development of a diagnostic method based on circle-to-circle amplification (C2CA) and oligonucleotide-functionalized magnetic nanoparticles. Firstly, the performance of the detection method was evaluated in terms of sensitivity and speed. Synthetic target sequences for Newcastle disease virus and Salmonella were used as model sequences. The sensitivity of the C2CA assay resulted in detection of 1 amol of starting DNA target with a total amplification time of 40 min for both target sequences. Secondly, the functionalization of the nanoparticles was evaluated in terms of robustness and stability. The functionalization was shown to be very robust, and the stability test showed that 92% of the oligos were still attached on the particle surface after three months of storage at 4 °C. Altogether, the results obtained in this study provide a strong foundation for the development of a quick and sensitive diagnostic assay.
本工作探讨了基于循环循环扩增（C2CA）和寡核苷酸功能化磁性纳米粒子开发诊断方法的几个重要问题。首先，从灵敏度和速度两方面评估了检测方法的性能。新城疫病毒和沙门氏菌的合成靶序列被用作模型序列。C2CA 分析的灵敏度可检测起始 DNA 靶标 1 飞摩尔，两种靶序列的总扩增时间为 40 分钟。其次，从稳健性和稳定性两方面评估了纳米粒子的功能化。结果表明，功能化非常稳健，稳定性测试表明，在 4°C 下储存三个月后，仍有 92%的寡核苷酸附着在颗粒表面。总之，本研究获得的结果为快速灵敏的诊断测定法的开发提供了坚实的基础。
Zotero 插件