Marchand Jérémy, Guitton Yann, Martineau Estelle, Royer Anne-Lise, Balgoma David, Le Bizec Bruno, Giraudeau Patrick, Dervilly Gaud
LABERCA, Oniris, INRAE, 44307 Nantes, France.
CEISAM UMR 6230, Université de Nantes, CNRS, 44000 Nantes, France.
Foods. 2021 May 28;10(6):1218. doi: 10.3390/foods10061218.
From a general public health perspective, a strategy combining non-targeted and targeted lipidomics MS-based approaches is proposed to identify disrupted patterns in serum lipidome upon growth promoter treatment in pigs. Evaluating the relative contributions of the platforms involved, the study aims at investigating the potential of innovative analytical approaches to highlight potential chemical food safety threats. Serum samples collected during an animal experiment involving control and treated pigs, whose food had been supplemented with ractopamine, were extracted and characterised using three MS strategies: Non-targeted RP LC-HRMS; the targeted Lipidyzer™ platform (differential ion mobility associated with shotgun lipidomics) and a homemade LC-HRMS triglyceride platform. The strategy enabled highlighting specific lipid profile patterns involving various lipid classes, mainly in relation to cholesterol esters, sphingomyelins, lactosylceramide, phosphatidylcholines and triglycerides. Thanks to the combination of non-targeted and targeted MS approaches, various compartments of the pig serum lipidome could be explored, including commonly characterised lipids (Lipidyzer™), triglyceride isomers (Triglyceride platform) and unique lipid features (non-targeted LC-HRMS). Thanks to their respective characteristics, the complementarity of the three tools could be demonstrated for public health purposes, with enhanced coverage, level of characterization and applicability.
从公共卫生的总体角度出发，我们提出了一种结合非靶向和靶向脂质组学质谱分析方法的策略，以识别猪在生长促进剂处理后血清脂质组中被破坏的模式。通过评估所涉及平台的相对贡献，该研究旨在探究创新分析方法突出潜在化学食品安全威胁的潜力。在一项动物实验中，收集了对照组和经莱克多巴胺处理组猪的血清样本，其食物中添加了莱克多巴胺，并使用三种质谱策略对样本进行提取和表征：非靶向反相液相色谱-高分辨质谱；靶向Lipidyzer™平台（与鸟枪法脂质组学相关的差分离子迁移率）和自制的液相色谱-高分辨质谱甘油三酯平台。该策略能够突出涉及各种脂质类别的特定脂质谱模式，主要与胆固醇酯、鞘磷脂、乳糖神经酰胺、磷脂酰胆碱和甘油三酯有关。由于非靶向和靶向质谱方法的结合，可以探索猪血清脂质组的各个部分，包括常见的特征脂质（Lipidyzer™）、甘油三酯异构体（甘油三酯平台）和独特的脂质特征（非靶向液相色谱-高分辨质谱）。由于它们各自的特性，这三种工具的互补性可用于公共卫生目的，具有更高的覆盖范围、表征水平和适用性。
