人腺病毒2型早期区域2B的mRNA的精确结构和编码能力。

The precise structure and coding capacity of mRNAs from early region 2B of human adenovirus serotype 2.

作者信息

Shu L, Pettit S C, Engler J A

机构信息

Department of Biochemistry, University of Alabama, Birmingham 35294.

出版信息

Virology. 1988 Aug;165(2):348-56. doi: 10.1016/0042-6822(88)90579-x.

DOI:10.1016/0042-6822(88)90579-x

PMID:3407147

Abstract

Replication of human adenovirus (Ad) DNA requires three virus-encoded proteins that are coordinately transcribed from a single promoter at early times after infection. The mRNAs for two of these proteins, the precursor to the terminal protein (pTP) and the Ad DNA polymerase (Ad Pol), share several exons, including one encoded near Ad genome coordinate 39. The positions of the splice points of these mRNAs have been mapped by S1 nuclease mapping, by RNA sequencing, and by cDNA cloning. As a result of RNA splicing events, a short open reading frame (ORF) encoded at genome coordinate 39 is connected to the beginning of both the pTP and Ad Pol coding sequences; inclusion of this upstream ORF is essential for expression of functional pTP and Ad Pol proteins.

摘要

人腺病毒（Ad）DNA的复制需要三种病毒编码蛋白，这些蛋白在感染后的早期从单个启动子协同转录。其中两种蛋白的mRNA，即末端蛋白前体（pTP）和Ad DNA聚合酶（Ad Pol），共享几个外显子，包括一个在Ad基因组坐标39附近编码的外显子。这些mRNA的剪接位点位置已通过S1核酸酶图谱分析、RNA测序和cDNA克隆进行了定位。由于RNA剪接事件，在基因组坐标39处编码的一个短开放阅读框（ORF）与pTP和Ad Pol编码序列的起始部分相连；包含这个上游ORF对于功能性pTP和Ad Pol蛋白的表达至关重要。