Silver L, Anderson C W
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Virology. 1988 Aug;165(2):377-87. doi: 10.1016/0042-6822(88)90582-x.
Human adenoviruses (e.g., Ad2, Ad5) establish chronic infections in human lymphoid-derived cell lines, including Raji and Jijoye (R.E. Wallace, 1969, Proc. Soc. Exp. Biol. Med. 130, 702-710; N. Faucon, G. Ogier, and Y. Chardonnet, 1982, J. Natl. Cancer Inst. 69, 1215-1220); however, the mechanisms by which chronic infections are established and maintained are not understood. When Raji or MOLT-3 cell cultures were infected with Ad2 at high multiplicity, these cell lines continued to grow exponentially and produced only small amounts of infectious virus. Virus-specific antigens, including the DNA-binding protein and hexon, were expressed in only 5% of the Ad2-inoculated cultures. All Raji and MOLT-3 cells were found to have adenovirus receptors, but the Ad2 virions that adsorbed to most Raji cells were sequestered in caps, suggesting that most cells fail to internalize adsorbed Ad2. Cell synchronization experiments showed a correlation between the proportion of cells that became productively infected and the proportion of cells in mitosis at the time of infection. In contrast, primary blood lymphocytes had few, if any, Ad2 receptors and were not productively infected by Ad2.
人类腺病毒（如Ad2、Ad5）可在人类淋巴来源的细胞系中建立慢性感染，包括Raji和Jijoye细胞系（R.E. Wallace，1969年，《实验生物学与医学学会会刊》130卷，702 - 710页；N. Faucon、G. Ogier和Y. Chardonnet，1982年，《国家癌症研究所杂志》69卷，1215 - 1220页）；然而，建立和维持慢性感染的机制尚不清楚。当Raji或MOLT - 3细胞培养物以高感染复数感染Ad2时，这些细胞系继续呈指数生长，仅产生少量感染性病毒。病毒特异性抗原，包括DNA结合蛋白和六邻体，仅在5%的接种Ad2的培养物中表达。所有Raji和MOLT - 3细胞都被发现具有腺病毒受体，但吸附到大多数Raji细胞上的Ad2病毒粒子被隔离在包涵体中，这表明大多数细胞未能内化吸附的Ad2。细胞同步化实验表明，发生有效感染的细胞比例与感染时处于有丝分裂期的细胞比例之间存在相关性。相比之下，原代血液淋巴细胞即使有也很少有Ad2受体，并且不会被Ad2有效感染。
