Horvath J, Palkonyay L, Weber J
J Virol. 1986 Jul;59(1):189-92. doi: 10.1128/JVI.59.1.189-192.1986.
Human peripheral blood lymphocytes from healthy adults, cord blood lymphocytes, and lymphoblastoid cell lines were screened by hybridization for the presence of group C adenovirus DNA sequences. In 13 of 17 peripheral blood lymphocyte samples from adults, 1 of 10 cord blood samples, and seven of seven lymphoblastoid cell lines tested, results were positive for Group C adenovirus DNA (adenovirus 1 [Ad1], Ad2, Ad5, or Ad6). About 1 to 2% of the lymphocytes carried 50 to 100 viral genome copies per positive cell, as estimated by in situ hybridization. Infectious virus representing all members of group C were recovered, but cultivation in the presence of adenovirus antibody did not cure the cells of free viral genomes. Viral DNA was found in B, T, and N cells but only in 1 of 10 cord blood samples. The results suggest that group C adenovirus infections in childhood result in the persistence of the viral genome in circulating lymphocytes.
通过杂交筛选健康成年人的人外周血淋巴细胞、脐血淋巴细胞和成淋巴细胞系，以检测C组腺病毒DNA序列的存在。在检测的17份成人外周血淋巴细胞样本中的13份、10份脐血样本中的1份以及7份成淋巴细胞系中的7份中，C组腺病毒DNA（腺病毒1 [Ad1]、Ad2、Ad5或Ad6）检测结果呈阳性。通过原位杂交估计，约1%至2%的淋巴细胞每个阳性细胞携带50至100个病毒基因组拷贝。代表C组所有成员的传染性病毒被回收，但在腺病毒抗体存在的情况下培养并不能使细胞清除游离病毒基因组。在B细胞、T细胞和N细胞中发现了病毒DNA，但仅在10份脐血样本中的1份中发现。结果表明，儿童期C组腺病毒感染会导致病毒基因组在循环淋巴细胞中持续存在。
