Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Arthropod-Borne Animal Diseases Research Unit, United States Department of Agriculture-Agricultural Research Service, Manhattan, KS 66502, USA.
Viruses. 2021 May 28;13(6):1016. doi: 10.3390/v13061016.
Bluetongue virus (BTV) is a segmented RNA virus transmitted by midges. Climatic factors, animal movement, vector species, and viral mutation and reassortment may all play a role in the occurrence of BTV outbreaks among susceptible ruminants. We used two enzootic strains of BTV (BTV-2 and BTV-10) to explore the potential for , a key North American vector, to be infected with these viruses, and identify the impact of temperature variations on virogenesis during infection. While BTV-10 replicated readily in following an infectious blood meal, BTV-2 was less likely to result in productive infection at biologically relevant exposure levels. Moreover, when were co-exposed to both viruses, we did not detect reassortment between the two viruses, despite previous in vitro findings indicating that BTV-2 and BTV-10 are able to reassort successfully. These results highlight that numerous factors, including vector species and exposure dose, may impact the in vivo replication of varying BTV strains, and underscore the complexities of BTV ecology in North America.
蓝舌病毒（BTV）是一种由蠓传播的分段 RNA 病毒。气候因素、动物运动、媒介物种以及病毒突变和重配都可能在易感反刍动物中 BTV 爆发的发生中起作用。我们使用两种地方性 BTV（BTV-2 和 BTV-10）来探索，一种北美的重要媒介，是否可能感染这些病毒，并确定温度变化对感染过程中病毒发生的影响。虽然 在摄入感染性血液后，BTV-10 可以很容易地复制，但在具有生物学意义的暴露水平下，BTV-2 不太可能导致有生产力的感染。此外，当 同时接触这两种病毒时，尽管之前的体外研究结果表明 BTV-2 和 BTV-10 能够成功重配，但我们没有检测到两种病毒之间的重配。这些结果突出表明，包括媒介物种和暴露剂量在内的许多因素可能会影响不同 BTV 株在体内的复制，并强调了 BTV 生态学在北美的复杂性。
