对……中抗氧化剂抗性基因的全基因组筛选 。（原文句子不完整，翻译可能不太准确，完整准确的翻译需补充完整原文）
Genome-Wide Screening of Oxidizing Agent Resistance Genes in .
作者信息
Chen Hao, Wilson Jessica, Ercanbrack Carson, Smith Hannah, Gan Qinglei, Fan Chenguang
机构信息
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA.
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA.
出版信息
Antioxidants (Basel). 2021 May 27;10(6):861. doi: 10.3390/antiox10060861.
The use of oxidizing agents is one of the most favorable approaches to kill bacteria in daily life. However, bacteria have been evolving to survive in the presence of different oxidizing agents. In this study, we aimed to obtain a comprehensive list of genes whose expression can make cells resistant to different oxidizing agents. For this purpose, we utilized the ASKA library and performed a genome-wide screening of ~4200 genes. Hydrogen peroxide (HO) and hypochlorite (HOCl) were tested as representative oxidizing agents in this study. To further validate our screening results, we used different strains as host cells to express or inactivate selected resistance genes individually. More than 100 genes obtained in this screening were not known to associate with oxidative stress responses before. Thus, this study is expected to facilitate both basic studies on oxidative stress and the development of antibacterial agents.
使用氧化剂是日常生活中杀灭细菌最有利的方法之一。然而，细菌一直在进化以在不同氧化剂存在的情况下存活。在本研究中，我们旨在获得一份基因综合列表，其表达可使细胞对不同氧化剂产生抗性。为此，我们利用了ASKA文库并对约4200个基因进行了全基因组筛选。本研究中测试了过氧化氢（HO）和次氯酸盐（HOCl）作为代表性氧化剂。为了进一步验证我们的筛选结果，我们使用不同菌株作为宿主细胞来单独表达或灭活选定的抗性基因。此次筛选中获得的100多个基因以前并不知晓与氧化应激反应相关。因此，本研究有望促进氧化应激的基础研究以及抗菌剂的开发。
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