INFN-Laboratori Nazionali del Gran Sasso, 67100 Assergi, Italy.
Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza-Università di Roma, 00185 Rome, Italy.
Int J Mol Sci. 2021 May 27;22(11):5745. doi: 10.3390/ijms22115745.
During spermatogenesis, the Golgi apparatus serves important roles including the formation of the acrosome, which is a sperm-specific organelle essential for fertilization. We have previously demonstrated that ATP-dependent Citrate Lyase (ATPCL) is required for spindle organization, cytokinesis, and fusome assembly during male meiosis, mainly due to is activity on fatty acid biosynthesis. Here, we show that depletion of also affects the organization of acrosome and suggest a role for this enzyme in the assembly of Golgi-derived structures during Drosophila spermatogenesis.
在精子发生过程中，高尔基体发挥着重要作用，包括形成顶体，这是一种对于受精至关重要的精子特异性细胞器。我们之前已经证明，ATP 依赖性柠檬酸裂解酶（ATPCL）对于雄性减数分裂过程中的纺锤体组织、胞质分裂和融合体组装是必需的，主要是由于其在脂肪酸生物合成中的活性。在这里，我们表明 ATPCL 的缺失也会影响顶体的组织，并表明该酶在果蝇精子发生过程中参与了高尔基体衍生结构的组装。
