Centre for Agriculture and the Bioeconomy (CAB), Queensland University of Technology (QUT), Brisbane, Queensland 4001, Australia.
Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, P.O. 30709, Nairobi 00100, Kenya.
Int J Mol Sci. 2021 May 31;22(11):5941. doi: 10.3390/ijms22115941.
Pigeonpea [ (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3'-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.
兵豆 [(L.) Millspaugh] 是一种经济上重要的豆科植物，在半干旱热带地区发挥着关键作用。兵豆易受 [Hübner] 的影响，这会导致严重的产量损失。这种害虫对许多商业上可用的杀虫剂产生了抗性。因此，兵豆的野生近缘种被认为是扩展栽培兵豆遗传基础的潜在基因来源，以改善对害虫和病原体的寄主植物抗性等特性。使用串联质量标签平台进行了定量蛋白质组学分析，以鉴定 IBS 3471 和 ICPL 87 耐病品种与 分别敏感品种之间差异丰度的蛋白质。在营养生长和开花/结荚生长阶段分析了叶片蛋白质组。IBS 3471 的 耐受性似乎与增强的防御反应有关，例如次生代谢前体、抗氧化剂和苯丙烷途径的变化。用 IBS 3471 冻干叶片的人工饲料饲养的幼虫的发育表现出与用含有 32 mg/25 g 人工饲料的槲皮素浓度的人工饲料饲养的幼虫相似的抑制作用。DAB 染色（3,3'-二氨基联苯胺）显示 IBS 3471 中活性氧的快速积累。我们得出结论，IBS 3471 是改善栽培兵豆遗传基础的理想候选者，包括对寄主植物抗性的特性。
