State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling 712100, China.
Int J Mol Sci. 2021 May 31;22(11):5942. doi: 10.3390/ijms22115942.
Plant proline-rich proteins (PRPs) are cell wall proteins that occur in the plant kingdom and are involved in plant development and stress response. In this study, 9 genes were identified from the apple genome and a comprehensive analysis of the family was conducted, including gene structures, phylogenetic analysis, chromosome mapping, and so on. The expression of varied among tissues and in response to different types of stresses. and were induced by five detected stress treatments, including heat, drought, abscisic acid, cold, and salt; the expression patterns of the others varied under different types of stress. Subcellular localization showed that MdPRPs mainly functioned in the cytoplasm, except for MdPRP1 and MdPRP5, which also functioned in the nucleus. When was overexpressed in tobacco, the transgenic plants showed higher tolerance to high temperature (48 °C) compared with wild-type (WT) plants. The transgenic plants showed milder wilting, a lower accumulation of electrolyte leakage, MDA and ROS, and a higher level of chlorophyll and SOD and POD activity, indicating that may be an important gene in apples for heat stress tolerance. Overall, this study suggested that MdPRPs are critically important for the ability of apple responses to stresses.
植物脯氨酸丰富蛋白（PRPs）是一种存在于植物界的细胞壁蛋白，参与植物的发育和应激反应。本研究从苹果基因组中鉴定了 9 个基因，并对 家族进行了全面分析，包括基因结构、系统发育分析、染色体定位等。在不同组织中表达不同，并对不同类型的胁迫有反应。、和 被五种检测到的胁迫处理（热、干旱、脱落酸、冷和盐）诱导；其他的表达模式在不同类型的胁迫下有所不同。亚细胞定位表明 MdPRPs 主要在细胞质中发挥作用，除了 MdPRP1 和 MdPRP5，它们还在核中发挥作用。当 在烟草中过表达时，与野生型（WT）植物相比，转基因植物对高温（48°C）表现出更高的耐受性。转基因植物表现出较轻的萎蔫，电解质泄漏、MDA 和 ROS 的积累较低，叶绿素和 SOD 和 POD 活性较高，表明 可能是苹果耐热的重要基因。总的来说，本研究表明 MdPRPs 对苹果应对胁迫的能力至关重要。
