Biopolymer Analytics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25 Building 20, 14476 Potsdam-Golm, Germany.
Int J Mol Sci. 2021 May 26;22(11):5666. doi: 10.3390/ijms22115666.
Transitory starch granules result from complex carbon turnover and display specific situations during starch synthesis and degradation. The fundamental mechanisms that specify starch granule characteristics, such as granule size, morphology, and the number per chloroplast, are largely unknown. However, transitory starch is found in the various cells of the leaves of , but comparative analyses are lacking. Here, we adopted a fast method of laser confocal scanning microscopy to analyze the starch granules in a series of Arabidopsis mutants with altered starch metabolism. This allowed us to separately analyze the starch particles in the mesophyll and in guard cells. In all mutants, the guard cells were always found to contain more but smaller plastidial starch granules than mesophyll cells. The morphological properties of the starch granules, however, were indiscernible or identical in both types of leaf cells.
暂态淀粉粒是复杂碳代谢的结果，在淀粉合成和降解过程中表现出特定的情况。指定淀粉粒特征（如颗粒大小、形态和每个叶绿体的数量）的基本机制在很大程度上尚不清楚。然而，暂态淀粉存在于 的各种叶片细胞中，但缺乏比较分析。在这里，我们采用激光共聚焦扫描显微镜的快速方法来分析淀粉代谢改变的拟南芥突变体中的淀粉粒。这使我们能够分别分析叶肉细胞和保卫细胞中的淀粉颗粒。在所有突变体中，保卫细胞总是含有比叶肉细胞更多但更小的质体淀粉粒。然而，淀粉粒的形态特性在两种类型的叶细胞中是不可区分的或相同的。
