Mitchell Jordan L, O'Halloran Conor, Stanley Paul, McDonald Kieran, Burr Paul, Gunn-Moore Danièlle A, Hope Jayne C
The Royal (Dick) School of Veterinary Studies and The Roslin Institute, Easter Bush Campus, University of Edinburgh, Midlothian EH25 9RG, UK.
Biobest Laboratories Ltd., Edinburgh Technopole, Milton Bridge EH26 0PY, UK.
Pathogens. 2021 May 26;10(6):657. doi: 10.3390/pathogens10060657.
The interferon-gamma release assay (IGRA) is used to diagnose cases of feline mycobacteriosis, but the use of serial testing to monitor treatment responses has not been evaluated in this species. From a population of cats that underwent IGRA testing for diagnostic investigation, individuals were identified with a pre- and end-of-treatment IGRA that passed control thresholds. The number of cats which reverted to negative at the end-of-treatment IGRA, changes in paired antigen-specific optical density (OD) values and differences in the pre-treatment antigen-specific OD values for those which underwent reversion were compared. Factors to explain reversion or recurrence of disease post-treatment were explored. Four of 18 cats (22%) reverted to negativity at the point of clinical resolution ( = 0.33), there was no difference in paired antigen-specific OD values ( ≥ 0.12), and cats that reverted did not have a lower baseline OD value ( = 0.63). No statistically significant factors were identified to predict reversion ( ≥ 0.08). Remaining positive at the end of treatment IGRA was not associated with recurrence of disease post-treatment ( = 0.34). Overall, these data suggest there is limited value in the use of the IGRA to monitor treatment responses in cats.
干扰素-γ释放试验（IGRA）用于诊断猫分枝杆菌病病例，但尚未在该物种中评估使用系列检测来监测治疗反应的情况。从接受IGRA检测以进行诊断调查的猫群中，识别出治疗前和治疗结束时IGRA通过对照阈值的个体。比较了治疗结束时IGRA恢复为阴性的猫的数量、配对抗原特异性光密度（OD）值的变化以及恢复的猫的治疗前抗原特异性OD值的差异。探讨了解释治疗后疾病恢复或复发的因素。18只猫中有4只（22%）在临床缓解时恢复为阴性（ = 0.33），配对抗原特异性OD值无差异（ ≥ 0.12），恢复的猫的基线OD值并不更低（ = 0.63）。未发现有统计学意义的因素可预测恢复情况（ ≥ 0.08）。治疗结束时IGRA仍为阳性与治疗后疾病复发无关（ = 0.34）。总体而言，这些数据表明，使用IGRA监测猫的治疗反应价值有限。
