Alimu Ayiguli, Abudureman Haiqiemuhan, Li Meiyan, Wang Yongzhi, Wang Jiasui, Jiang Shurui, Wang Menglin, Liu Zaoling
School of Public Health, Xinjiang Medical University, Urumqi 830011, China.
Wei Sheng Yan Jiu. 2021 May;50(3):476-482. doi: 10.19813/j.cnki.weishengyanjiu.2021.03.022.
To investigate the effect of decabromodiphenyl ether(BDE-209) on glucose and lipid metabolism and adipocytokines in mice.
A total of 36 adult male C57 BL/6 mice were randomly divided into 6 groups, 1000, 800, 600, 450 and 300 mg/kg groups and the control group, with 6 in each group. 60 days after gavage, fasting overnight, the mice were killed and the corresponding test materials were taken. Using an automatic biochemical analyzer to detect triglycerides(TG), total cholesterol(cholesterol, TC), low-density lipoprotein(LDL-C), and high-density lipoprotein(HDL-C). Detected rat fasting blood glucose(FBG) with Roche blood glucose meter. Observed the morphological changes of mouse liver tissue using HE staining. Determined leptin(LEP), the mRNA and protein expression levels of adiponectin(ADP) and peroxisome proliferators-activated receptors(PPAR)-γ with Real-time fluorescent quantitative PCR and Western blotting.
The levels of fasting serum lisulin(FINS), insulin sensitivity index(ISI), ADP and HDL-C in the serum of the BDE-209 exposure group decreased(P& lt; 0. 05). The levels of FBG, LEP, TC, TG and LDL-C in the serum of the BDE-209 exposure group increased(P& lt; 0. 05). mRNA and protein expression levels of LEP and PPAR-γ in the liver increased(P& lt; 0. 05), and ADP mRNA and protein expression levels decreased(P& lt; 0. 05). Adipocyte factor LEP was positively correlated with the content of FBG, TG, TC and LDL-C, with r values of 0. 775, 0. 767, 0. 716 and 0. 812(P& lt; 0. 05), and negatively correlated with FINS and HDL-C, with r values of-0. 919 and-0. 817(P& lt; 0. 05). ADP was positively correlated with FINS and HDL-C, with r values of 0. 824 and 0. 832(P& lt; 0. 05), and negatively correlated with FBG, TG, TC and LDL-C, with r values of-0. 883, -0. 686, -0. 704 and-0. 772, respectively(P& lt; 0. 05). BDE-209 exposure to each dose group could change the morphology of mouse liver tissue to a certain extent.
Decabromodiphenyl ether can disturb the metabolism of glucose and lipids. Among them, the disorder in the high and high dose groups of BDE-209 is more obvious. The low dose of BDE-209 can also change the morphology of liver tissue.
探讨十溴二苯醚（BDE - 209）对小鼠糖脂代谢及脂肪细胞因子的影响。
将36只成年雄性C57 BL/6小鼠随机分为6组，即1000、800、600、450和300 mg/kg组以及对照组，每组6只。灌胃60天后，小鼠隔夜禁食，处死后取相应检测材料。使用自动生化分析仪检测甘油三酯（TG）、总胆固醇（cholesterol，TC）、低密度脂蛋白（LDL - C）和高密度脂蛋白（HDL - C）。用罗氏血糖仪检测大鼠空腹血糖（FBG）。采用HE染色观察小鼠肝脏组织形态学变化。用实时荧光定量PCR和蛋白质免疫印迹法测定瘦素（LEP）、脂联素（ADP）和过氧化物酶体增殖物激活受体（PPAR）-γ的mRNA和蛋白表达水平。
BDE - 209暴露组血清空腹胰岛素（FINS）、胰岛素敏感指数（ISI）、ADP和HDL - C水平降低（P＜0.05）。BDE - 209暴露组血清FBG、LEP、TC、TG和LDL - C水平升高（P＜0.05）。肝脏中LEP和PPAR -γ的mRNA和蛋白表达水平升高（P＜0.05），而ADP的mRNA和蛋白表达水平降低（P＜0.05）。脂肪细胞因子LEP与FBG、TG、TC和LDL - C含量呈正相关，r值分别为0.775、0.767、0.716和0.812（P＜0.05），与FINS和HDL - C呈负相关，r值分别为 - 0.919和 - 0.817（P＜0.05）。ADP与FINS和HDL - C呈正相关，r值分别为0.824和0.832（P＜0.05），与FBG、TG、TC和LDL - C呈负相关，r值分别为 - 0.883、 - 0.686、 - 0.704和 - 0.772（P＜0.05）。各剂量组BDE - 209暴露均可在一定程度上改变小鼠肝脏组织形态。
十溴二苯醚可扰乱糖脂代谢。其中，BDE - 209高剂量组和中剂量组的紊乱更为明显。低剂量的BDE - 209也可改变肝脏组织形态。
