Taira M, Kohno S W, Yamamura H, Ohata K
Department of Pharmacology, Kyoto Pharmaceutical University, Japan.
Agents Actions. 1988 Jun;24(1-2):189-95. doi: 10.1007/BF01968100.
Possible chemical mediators contributing to 48 hour passive cutaneous anaphylaxis (PCA) in rats were investigated. Forty-eight hour PCA was inhibited considerably by mepyramine and methysergide given intravenously, a finding suggestive of a major role for histamine and serotonin in the reaction. AA-861, a selective 5-lipoxygenase inhibitor did not inhibit the PCA, and leukotriene (LT)D4 or LTE4 and the combination with prostaglandin (PG)E2 had no significant skin reaction. In addition, only small amounts of slow reacting substance of anaphylaxis (SRS-A) were detected in skin fragments, in vitro. Although CV-3988, a selective platelet activating factor (PAF) antagonist, dose-dependently inhibited the PAF-induced skin reaction, the PCA was not affected by treatment with this compound. Indomethacin also had no inhibitory activity on PCA. Thus, sulfidopeptide LTs, PAF and arachidonate cyclooxygenase metabolites probably do not contribute to PCA, at least in rats.
对可能导致大鼠48小时被动皮肤过敏反应（PCA）的化学介质进行了研究。静脉注射美吡拉敏和甲基麦角新碱可显著抑制48小时PCA，这一发现表明组胺和5-羟色胺在该反应中起主要作用。选择性5-脂氧合酶抑制剂AA-861不抑制PCA，白三烯（LT）D4或LTE4以及与前列腺素（PG）E2的组合均无明显皮肤反应。此外，体外仅在皮肤碎片中检测到少量过敏反应慢反应物质（SRS-A）。尽管选择性血小板活化因子（PAF）拮抗剂CV-3988剂量依赖性地抑制PAF诱导的皮肤反应，但该化合物处理对PCA无影响。吲哚美辛对PCA也无抑制活性。因此，硫肽白三烯、PAF和花生四烯酸环氧化酶代谢产物可能至少在大鼠中对PCA无作用。
