Eckert R, Repke H, Rentz E, Nausch M, Friemel H
Institute of Clinical Immunology, Humboldt University, Charité, Berlin, German Democratic Republic.
Agents Actions. 1988 Jun;24(1-2):87-94. doi: 10.1007/BF01968084.
Mouse spleen cells were incubated for 24 hours in the presence of histamine (10(-13)-10(-3) M). Aliquots of the histamine free supernatants were intravenously injected into either syngenic or allogenic mice which were subsequently analysed by the Jerne plaque assay in respect of their specific IgM response against red blood cells from sheep. Depending on the histamine concentration during the preincubation and the mouse strain, the effects of the supernatants were found to be suppressive or potentiating. Both the histamine induced suppressor and potentiating factor are generated by T-cells and can be precipitated by (NH4)2SO4. These precipitates were fractionated by gel filtration which revealed a molecular weight of both factors in the range of 20 to 50 kDa. Both lymphokines were found to be sensitive to trypsin, low pH, and heat treatment but were not affected by neuraminidase. They do only interfere with the early events of the IgM response within 24 hours after the administration of the antigen. The conclusion is that the bidirectional modulation of the humoral immune response by histamine in mice is partially due to the induction of these lymphokines by a different efficacy of the histamine receptor activation at different T-cell subsets.
将小鼠脾细胞在组胺（10⁻¹³ - 10⁻³ M）存在的情况下孵育24小时。将无组胺上清液的等分试样静脉注射到同基因或异基因小鼠体内，随后通过耶尔恩斑试验分析它们针对绵羊红细胞的特异性IgM反应。根据预孵育期间的组胺浓度和小鼠品系，发现上清液的作用具有抑制性或增强性。组胺诱导的抑制因子和增强因子均由T细胞产生，并且可以被硫酸铵沉淀。通过凝胶过滤对这些沉淀物进行分级分离，结果显示这两种因子的分子量在20至50 kDa范围内。发现这两种淋巴因子对胰蛋白酶、低pH和热处理敏感，但不受神经氨酸酶影响。它们仅在给予抗原后24小时内干扰IgM反应的早期事件。结论是，组胺对小鼠体液免疫反应的双向调节部分归因于组胺受体在不同T细胞亚群上的不同激活效力诱导了这些淋巴因子。
