Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor - IPVDF, Eldorado do Sul, RS, Brasil.
Instituto de Biociências, Universidade de São Paulo - USP, São Paulo, SP, Brasil.
Rev Bras Parasitol Vet. 2021 May 28;30(2):e000421. doi: 10.1590/S1984-29612021043. eCollection 2021.
Anaplasma marginale is a vector-borne pathogen that causes a disease known as anaplasmosis. No sequenced genomes of Brazilian strains are yet available. The aim of this work was to compare whole genomes of Brazilian strains of A. marginale (Palmeira and Jaboticabal) with genomes of strains from other regions (USA and Australia strains). Genome sequencing of Brazilian strains was performed by means of next-generation sequencing. Reads were mapped using the genome of the Florida strain of A. marginale as a reference sequence. Single nucleotide polymorphisms (SNPs) and insertions/deletions (INDELs) were identified. The data showed that two Brazilian strains grouped together in one particular clade, which grouped in a larger American group together with North American strains. Moreover, some important differences in surface proteins between the two Brazilian isolates can be discerned. These results shed light on the evolutionary history of A. marginale and provide the first genome information on South American isolates. Assessing the genome sequences of strains from different regions is essential for increasing knowledge of the pan-genome of this bacteria.
边缘无浆体是一种由节肢动物传播的病原体，可引起无浆体病。目前还没有巴西菌株的测序基因组。本工作旨在比较巴西边缘无浆体（Palmeira 和 Jaboticabal）菌株与来自其他地区（美国和澳大利亚）菌株的全基因组。通过下一代测序对巴西菌株进行基因组测序。使用佛罗里达州边缘无浆体菌株的基因组作为参考序列来映射读取。鉴定了单核苷酸多态性（SNP）和插入/缺失（INDEL）。数据表明，两种巴西菌株聚集在一个特定的分支中，该分支与北美的菌株一起聚集在一个更大的美洲群体中。此外，还可以区分两个巴西分离株在表面蛋白上的一些重要差异。这些结果阐明了边缘无浆体的进化历史，并提供了南美分离株的首个基因组信息。评估来自不同地区的菌株的基因组序列对于增加对该细菌泛基因组的了解至关重要。
