de la Fuente José, Ruybal Paula, Mtshali Moses S, Naranjo Victoria, Shuqing Li, Mangold Atilio J, Rodríguez Sergio D, Jiménez Rafael, Vicente Joaquín, Moretta Rosalía, Torina Alessandra, Almazán Consuelo, Mbati Peter M, de Echaide Susana Torioni, Farber Marisa, Rosario-Cruz Rodrigo, Gortazar Christian, Kocan Katherine M
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078-2007, USA.
Vet Microbiol. 2007 Jan 31;119(2-4):382-90. doi: 10.1016/j.vetmic.2006.09.015. Epub 2006 Nov 2.
Anaplasma marginale is a tick-borne pathogen of cattle that causes the disease bovine anaplasmosis worldwide. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. MSP1a is involved in the adhesion and transmission of A. marginale by ticks and varies among geographic strains in the number and sequence of amino-terminal tandem repeats. The aim of this study was to characterize the genetic diversity of A. marginale strains collected from countries in North and South America, Europe, Asia, Africa and Australia, inclusive of all continents. In this study, we characterized 131 strains of A. marginale using 79 MSP1a repeat sequences. These results corroborated the genetic heterogeneity of A. marginale strains in endemic regions worldwide. The phylogenetic analyses of MSP1a repeat sequences did not result in clusters according to the geographic origin of A. marginale strains but provided phylogeographic information. Seventy-eight percent of the MSP1a repeat sequences were present in strains from a single geographic region. Strong (> or =80%) support was found for clusters containing sequences from Italian, Spanish, Chinese, Argentinean and South American strains. The phylogenetic analyses of MSP1a repeat sequences suggested tick-pathogen co-evolution and provided evidence of multiple introductions of A. marginale strains from various geographic locations worldwide. These results contribute to the understanding of the genetic diversity and evolution of A. marginale and tick-pathogen interactions.
边缘无形体是一种通过蜱传播的牛病原体，在全球范围内引起牛无形体病。主要表面蛋白（MSP）参与宿主-病原体和蜱-病原体相互作用，并已被用作边缘无形体菌株遗传特征鉴定和系统发育研究的标记。MSP1a参与边缘无形体通过蜱的黏附和传播，并且在地理菌株之间，其氨基末端串联重复序列的数量和序列存在差异。本研究的目的是对从北美洲、南美洲、欧洲、亚洲、非洲和澳大利亚（涵盖所有大洲）各国收集的边缘无形体菌株的遗传多样性进行表征。在本研究中，我们使用79个MSP1a重复序列对131株边缘无形体进行了表征。这些结果证实了全球流行地区边缘无形体菌株的遗传异质性。MSP1a重复序列的系统发育分析并未根据边缘无形体菌株的地理来源形成聚类，但提供了系统地理信息。78%的MSP1a重复序列存在于来自单一地理区域的菌株中。发现对包含来自意大利、西班牙、中国、阿根廷和南美菌株序列的聚类有强烈（≥80%）支持。MSP1a重复序列的系统发育分析表明蜱-病原体共同进化，并提供了边缘无形体菌株从全球各地不同地理位置多次引入的证据。这些结果有助于理解边缘无形体的遗传多样性和进化以及蜱-病原体相互作用。
