巴西联邦区水豚（Hydrochoerus hydrochaeris）感染斑点热立克次体，巴西联邦区非巴西斑点热流行区。
Capybara (Hydrochoerus hydrochaeris) exposure to Rickettsia in the Federal District of Brazil, a non-endemic area for Brazilian spotted fever.
机构信息
Laboratório de Patologia Clínica Veterinária, Faculdade de Agronomia e Veterinária - FAV, Universidade de Brasília - UnB, Brasília, DF, Brasil.
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo - USP, São Paulo, SP, Brasil.
出版信息
Rev Bras Parasitol Vet. 2021 May 28;30(2):e028720. doi: 10.1590/S1984-29612021035. eCollection 2021.
In this study, whole blood samples and ticks were collected from 57 capybaras in recreational areas in the Federal District, Brazil, aiming to investigate the presence of Rickettsia spp. using polymerase chain reaction (PCR) and indirect immunofluorescence (IFAT) assays. None of the capybara blood samples yielded rickettsial DNA by PCR. Among 55 capybara serum samples tested by IFAT, 53 (96.3%) reacted to Rickettsia spp. Among these, 21 (39.6%) identified the R. bellii antigen as the probable antigen involved in a homologous reaction (PAIHR), whereas 2 (3.8%) identified the R. parkeri antigen. Ticks collected from capybaras were identified as 173 Amblyomma sculptum and 410 A. dubitatum, in addition to nine Amblyomma spp. larvae. A sample of 231 ticks was subjected to DNA extraction and PCR for Rickettsia species. None of 122 A. sculptum yielded rickettsial DNA. Molecular evidence of R. bellii was found in 25/108 (23.1%) and of Rickettsia sp. strain Cooperi (R. parkeri-like agent) in 2/108 (1.9%) of the A. dubitatum samples. These results suggest a greater exposure to R. bellii in these capybara populations, in addition to a more significant number of A. dubitatum, which might characterize the Federal District region as not endemic for Brazilian spotted fever.
在这项研究中，从巴西联邦区娱乐区的 57 只水豚中采集了全血样本和蜱虫，旨在使用聚合酶链反应（PCR）和间接免疫荧光（IFAT）检测法调查立克次体属的存在。PCR 未从任何水豚血液样本中获得立克次体 DNA。在通过 IFAT 检测的 55 份水豚血清样本中，有 53 份（96.3%）对立克次体属产生反应。在这些样本中，有 21 份（39.6%）鉴定为 R. bellii 抗原，可能是同源反应的相关抗原（PAIHR），而有 2 份（3.8%）鉴定为 R. parkeri 抗原。从水豚身上采集的蜱虫被鉴定为 173 只 A. sculptum 和 410 只 A. dubitatum，此外还有 9 只 A. spp.幼虫。采集了 231 只蜱虫进行 DNA 提取和 PCR 检测，以确定立克次体物种。122 只 A. sculptum 中没有一只产生立克次体 DNA。在 108 只 A. dubitatum 样本中发现了 25/108（23.1%）的 R. bellii 和 2/108（1.9%）的 Rickettsia sp. strain Cooperi（R. parkeri 样剂）。这些结果表明，这些水豚种群接触 R. bellii 的可能性更大，同时接触 A. dubitatum 的可能性也更大，这可能使联邦区区域不具有巴西斑点热的地方性。
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