Microbiology laboratory, Department of Zoology, GCU Lahore, Pakistan.
Fatima Memorial Hospital Lahore, Pakistan.
Braz J Biol. 2021 May 28;82:e228009. doi: 10.1590/1519-6984.228009. eCollection 2021.
Pseudomonas aeruginosa is a common opportunistic pathogenic bacterium with the ability to develop a strong communication pathway by quorum sensing system and different virulent factors. Among the various important secretions of P. aeruginosa rhamnolipid is important biological detergent, believed to be involved in the development of the biofilm and intercellular communication. It readily dissolves the lung surfactants that are then easily catalyzed by the phospholipases and in this way is involved in the acute pulmonary infection.
research work was designed to investigate virulence and gene associated with virulence in P. aeruginosa responsible for pulmonary infections.
In current study polymerase chain reaction (PCR) was used for the detection of the rhlR (rhamnolipid encoding) gene of isolated strains. A number of assays were performed that ensured its virulent behavior. Disc diffusion method was used to check its antibiotic resistance. Isolated strains were resistant to a number of antibiotics applied.
It was found that males are more prone to respiratory infections as compared to females. Male members with age of 44-58 and 59-73 are at a higher risk, while females with age of 44-58 are also at a risk of pulmonary infections. Antibiotic resistance was observed by measuring zone of inhibition in strains GCU-SG-M4, GCU-SG-M3, GCU-SG-M5, GCU-SG-M2, GCU-SG-M1 and GCU-SG-M6. GCU-SG-M2 was resistant to fluconazole (FLU), clarithromycin (CLR), cefixime (CFM) and Penicillin (P10). No zone of inhibition was observed. But it showed unusual diffused zone around the Ak and MEM antibiotic discs. rhl R gene and 16s rRNA gene were characterized and analyzed.
Findings from current study would help in raising awareness about antibiotic resistance of P. aeruginosa, and also the sequence of rhl R gene can be used as the diagnostic marker sequence to identify the virulent rhl R gene sequence from the samples when isolated from sputum of Pneumonia patients.
铜绿假单胞菌是一种常见的机会性病原体细菌，具有通过群体感应系统和不同毒力因子形成强大通讯途径的能力。在铜绿假单胞菌的各种重要分泌物中，鼠李糖脂是一种重要的生物清洁剂，据信它参与了生物膜的形成和细胞间通讯。它容易溶解肺表面活性剂，然后很容易被磷脂酶催化，从而参与急性肺部感染。
本研究旨在研究与肺部感染有关的铜绿假单胞菌的毒力和相关基因。
在目前的研究中，聚合酶链反应（PCR）用于检测分离株的 rhlR（鼠李糖脂编码）基因。进行了一系列检测以确保其毒力行为。采用圆盘扩散法检测其抗生素耐药性。分离株对应用的多种抗生素均有耐药性。
发现男性比女性更容易发生呼吸道感染。44-58 岁和 59-73 岁的男性成员风险更高，而 44-58 岁的女性也存在肺部感染的风险。通过测量菌株 GCU-SG-M4、GCU-SG-M3、GCU-SG-M5、GCU-SG-M2、GCU-SG-M1 和 GCU-SG-M6 的抑菌圈来观察抗生素耐药性。GCU-SG-M2 对氟康唑（FLU）、克拉霉素（CLR）、头孢克肟（CFM）和青霉素（P10）耐药。没有观察到抑菌圈。但它在 Ak 和 MEM 抗生素圆盘周围显示出异常弥散的区域。rhlR 基因和 16s rRNA 基因进行了特征和分析。
本研究的结果将有助于提高对铜绿假单胞菌抗生素耐药性的认识，并且 rhlR 基因的序列也可以作为诊断标记序列，用于从肺炎患者的痰样中识别毒力 rhlR 基因序列。
