Zhu Yue, Feng Yuan Zhang Wei Wei Jifeng
The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing, Jiangsu, China.
J BUON. 2021 Mar-Apr;26(2):353-358.
To explore the role and possible regulatory mechanisms of GRAIL in lung adenocarcinoma cells.
Online databases were used to analyze the expression and prognosis of GRAIL in patients with lung adenocarcinoma. Lung adenocarcinoma cells (A549 and H1299) were transfected with small interfering RNA or infected with lentivirus. The constructed cell models were validated by real-time fluorescence quantitative PCR. Cell proliferation ability was detected by CCK8 assay, cell migration and invasion ability were detected by scratch assay and Transwell assay. Suppressed hallmark gene sets of GRAIL in lung adenocarcinoma was performed by GeneSet Enrichment Analysis. The mRNA and protein level of C-MYC were detected by real-time fluorescence quantitative PCR and western blot. The protein level of STAT3 and phosphorylated STAT3 were detected by western blot.
GRAIL is poorly expressed in lung adenocarcinoma tissue. Down-regulation of GRAIL is associated with poor prognosis of lung adenocarcinoma patients. GRAIL expression is negatively correlated with the proliferation, migration, and invasion of lung adenocarcinoma cells. GRAIL inhibits activation of STAT3 and transcription of C-MYC.
GRAIL inhibits the growth, migration and invasion of lung adenocarcinoma cells. GRAIL might play an anti-cancer role through regulating STAT3/ C-MYC signaling pathways.
探讨GRAIL在肺腺癌细胞中的作用及可能的调控机制。
利用在线数据库分析GRAIL在肺腺癌患者中的表达及预后情况。用小干扰RNA转染肺腺癌细胞（A549和H1299）或用慢病毒感染。通过实时荧光定量PCR验证构建的细胞模型。采用CCK8法检测细胞增殖能力，划痕试验和Transwell试验检测细胞迁移和侵袭能力。通过基因集富集分析对肺腺癌中GRAIL的标志性基因集进行抑制分析。采用实时荧光定量PCR和蛋白质免疫印迹法检测C-MYC的mRNA和蛋白水平。采用蛋白质免疫印迹法检测STAT3和磷酸化STAT3的蛋白水平。
GRAIL在肺腺癌组织中低表达。GRAIL下调与肺腺癌患者预后不良相关。GRAIL表达与肺腺癌细胞的增殖、迁移和侵袭呈负相关。GRAIL抑制STAT3的激活和C-MYC的转录。
GRAIL抑制肺腺癌细胞的生长、迁移和侵袭。GRAIL可能通过调节STAT3/C-MYC信号通路发挥抗癌作用。
