Wang Meng, Ding Jian, Zhao Aihong, Zhang Yixin, Zhou Yongkun, Tian Zhaochun
The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
Department of General Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 16369, Jingshi Road, Jinan, Shandong, China.
Biochem Genet. 2024 Aug 15. doi: 10.1007/s10528-024-10895-2.
Colorectal cancer has the third highest incidence and second highest mortality rate among all cancer types. Exploring the molecular mechanisms driving malignant proliferation and metastasis of colorectal cancer will benefit the treatment and management of cancer patients. Recent studies have reported diametrically opposed roles of Ring finger protein 128 (RNF128) in different types of cancer. However, the role of RNF128 in colorectal cancer is still completely unknown, which this study attempts to analyze. The differential expression of RNF128 mRNA and protein in 30 pairs of colorectal cancer and corresponding peritumoral tissues was detected using RT-qPCR, western blot and immunohistochemical staining. siRNA specifically targeting RNF128 was transfected into colorectal cancer cell lines (SW1116 and SW480) cultured in vitro. Proliferation, growth, migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer cells were examined by CCK-8, clone formation, wound-healing, transwell, western blot and immunofluorescence assays. Both RNF128 mRNA and protein levels were significantly increased in colorectal cancer tissues compared to pericarcinoma tissues. Knockdown of RNF128 significantly inhibited the proliferation, growth, migration, invasion and EMT of SW480 and SW1116 cells. Targeting RNF128 may benefit the treatment and management of colorectal cancer.
在所有癌症类型中，结直肠癌的发病率位居第三，死亡率位居第二。探索驱动结直肠癌恶性增殖和转移的分子机制将有助于癌症患者的治疗和管理。最近的研究报道了环指蛋白128（RNF128）在不同类型癌症中发挥着截然相反的作用。然而，RNF128在结直肠癌中的作用仍完全未知，本研究试图对此进行分析。采用RT-qPCR、蛋白质免疫印迹和免疫组化染色检测30对结直肠癌组织及相应癌旁组织中RNF128 mRNA和蛋白的差异表达。将特异性靶向RNF128的小干扰RNA（siRNA）转染至体外培养的结直肠癌细胞系（SW1116和SW480）。通过CCK-8法、克隆形成实验、伤口愈合实验、Transwell实验、蛋白质免疫印迹和免疫荧光实验检测结直肠癌细胞的增殖、生长、迁移、侵袭及上皮-间质转化（EMT）情况。与癌旁组织相比，结直肠癌组织中RNF128 mRNA和蛋白水平均显著升高。敲低RNF128可显著抑制SW480和SW1116细胞的增殖、生长、迁移、侵袭及EMT。靶向RNF128可能有助于结直肠癌的治疗和管理。
