National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Phytopathology. 2022 Feb;112(2):452-459. doi: 10.1094/PHYTO-11-20-0498-R. Epub 2022 Jan 31.
The leaves of soybean cultivar ZheA8901 show various symptoms (necrosis, mosaic, and symptomless) when infected with different strains of soybean mosaic virus (SMV). Based on a proteomic analysis performed with tandem mass tags (TMT), 736 proteins were differentially expressed from soybean samples that showed asymptomatic, mosaic, and necrosis symptoms induced by SMV strains SC3, SC7, and SC15, respectively. Among these, GmGSTU13 and ascorbate peroxidase (APX) were only upregulated in mosaic and symptomless leaves, respectively. The protein level of GmGSTU13 determined by western blot analysis was consistent with TMT analysis, and quantitative reverse transcriptase PCR analysis showed that mRNA levels in mosaic plants were 5.26- and 3.75-fold higher than those in necrotic and symptomless plants, respectively. Additionally, the expression of the viral () gene was increased, and serious mosaic symptoms were observed in -overexpressing plants inoculated with all three SMV strains. These results showed that is associated with the development of SMV-induced mosaic symptoms in soybean and that APX is upregulated in symptomless leaves at both the transcriptional and protein levels. In gene-silenced soybean plants, the relative expression of the viral gene was 1.50, 7.59, and 1.30 times higher than in positive control plants inoculated with the three SMV strains, suggesting that the upregulation of APX may be associated with lack of symptoms in soybean infected with SMV. This work provides a useful dataset for identifying key proteins responsible for symptom development in soybean infected with different SMV strains.
感病大豆品种浙农 8901 的叶片在感染不同大豆花叶病毒（SMV）株系时表现出不同的症状（坏死、花叶和无症状）。基于串联质量标签（TMT）的蛋白质组学分析，从表现出由 SMV 株系 SC3、SC7 和 SC15 分别诱导的无症状、花叶和坏死症状的大豆样本中鉴定到 736 种差异表达蛋白。其中，GmGSTU13 和抗坏血酸过氧化物酶（APX）分别在花叶和无症状叶片中上调。通过 Western blot 分析测定的 GmGSTU13 蛋白水平与 TMT 分析一致，定量逆转录聚合酶链反应分析显示，花叶植株中的 mRNA 水平分别比坏死和无症状植株高 5.26 倍和 3.75 倍。此外，病毒（）基因的表达增加，并且在接种所有三种 SMV 株系的过表达植株中观察到严重的花叶症状。这些结果表明，与大豆感染 SMV 后花叶症状的发展有关，并且在无症状叶片中，APX 的转录和蛋白水平均上调。在沉默 GmGSTU13 基因的大豆植株中，病毒（）基因的相对表达量比接种三种 SMV 株系的阳性对照植株高 1.50、7.59 和 1.30 倍，表明 APX 的上调可能与大豆感染 SMV 后无症状有关。这项工作为鉴定感染不同 SMV 株系的大豆中与症状发展相关的关键蛋白提供了有用的数据集。
