Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
College of Forestry Hainan University, Haikou, China.
Microsc Res Tech. 2021 Dec;84(12):2890-2905. doi: 10.1002/jemt.23847. Epub 2021 Jun 2.
Pedicularis groenlandica is one of the most important medicinal plant of Deosai Plateau (Gilgit-Baltistan) Pakistan. The present study was aim to evaluate the micromorphological features, phytochemical screening and pharmacological potential of P. groenlandica by using multiple microscopic techniques. Six different solvents were used to prepare P. groenlandica extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, α-amylase inhibition assay was performed. Cytotoxicity was tested using brine shrimp assay. Anti-leishmanial via MTT assay. Disc-diffusion assay was used for protein kinase inhibitory, antibacterial and antifungal activities. Palyno-anatomical study showed significant variation for the authentication and correct identification of this highly therapeutic plant by using light and scanning electron microscopic techniques. All extracts were found rich in phytochemicals, significant amount of phenolic and flavonoid contents were found in methanol extract (PGM) 95.78 mg GAE/g and 66.90 mg QE/g. Highest DPPH scavenging potential with IC 88.65 μg/mL, total antioxidant capacity (60.33 mg AAE/g sample) and total reducing power (83.97 mg AAE/g) were found for PGM. Disc-diffusion method showed significant antibacterial and antifungal activities. Noticeable growth inhibition in L. tropica was displayed by n-hexane extract (IC 112 μg/mL). Brine shrimp with highest LD (67.65 μg/mL) in ethyl-acetate extract. Ethanol extract gives persuasive protein kinase inhibition (26 mm) against Streptomyces 85-E hyphae. Highest alpha-amylase inhibition (74.10%) was found in n-hexane extract. In conclusion, our findings scientifically support the ethno-medicinal and biological potential of P. groenlandica. In future, the plant needs to be explored for further identification and isolation of bioactive compounds to develop new drugs to treat several aliments.
高山嵩草是巴基斯坦吉尔吉特-巴尔蒂斯坦的德奥赛高原（Deosai Plateau）上最重要的药用植物之一。本研究旨在利用多种显微镜技术评估高山嵩草的微观形态特征、植物化学筛选和药理学潜力。使用六种不同的溶剂制备高山嵩草提取物。用比色法测定植物化学和抗氧化活性。为了研究抗糖尿病，进行了α-淀粉酶抑制试验。使用卤虫试验测试细胞毒性。通过 MTT 法进行抗利什曼原虫。通过圆盘扩散法测定蛋白激酶抑制、抗菌和抗真菌活性。花粉解剖学研究表明，通过使用光和扫描电子显微镜技术，对这种具有高度治疗作用的植物进行认证和正确鉴定具有显著变化。所有提取物均富含植物化学物质，甲醇提取物（PGM）中发现大量的酚类和类黄酮含量，分别为 95.78mgGAE/g 和 66.90mgQE/g。PGM 具有最高的 DPPH 清除能力，IC 88.65μg/mL，总抗氧化能力（60.33mgAAE/g 样品）和总还原能力（83.97mgAAE/g）。正己烷提取物对 L. tropica 显示出显著的抑菌和抗真菌活性。乙酸乙酯提取物对卤虫的 LD 最高（67.65μg/mL）。乙醇提取物对链霉菌 85-E 菌丝具有较强的蛋白激酶抑制作用（26mm）。正己烷提取物的α-淀粉酶抑制率最高（74.10%）。总之，我们的发现从科学上支持高山嵩草的民族医学和生物学潜力。在未来，需要进一步研究该植物，以鉴定和分离生物活性化合物，开发治疗多种疾病的新药。
