Braillon A, Brody M J
Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242.
Am J Physiol. 1988 Aug;255(2 Pt 1):G191-3. doi: 10.1152/ajpgi.1988.255.2.G191.
A simple new method is described for chronic cannulation of the portal vein in rats. A Silastic catheter (0.64 mm OD) with an internal metallic leader is directly inserted in the junction of the two small ileal veins. The catheter is advanced into the mesenteric vein to the portal vein. Then the leader is removed and the catheter tunneled out the back of the neck. This method allows for the measurement of portal vein pressure, direct injection of substances in the portal vein, or blood samples for at least 1 wk in intact unrestrained rats. Validation included the presence of elevated portal vein pressure in portal hypertensive rats compared with normal rats and the absence of clot development in the portal vein. This method should help provide a better understanding of hepatic physiology and pharmacology in normal and disease states.
描述了一种用于大鼠门静脉慢性插管的简单新方法。带有内部金属导丝的硅橡胶导管（外径0.64毫米）直接插入两条小肠静脉的交汇处。将导管推进肠系膜静脉至门静脉。然后取出导丝，将导管从颈部后方引出。该方法能够在完整未束缚的大鼠中测量门静脉压力、直接向门静脉注射物质或采集血样，至少持续1周。验证包括与正常大鼠相比门静脉高压大鼠门静脉压力升高，以及门静脉内无血栓形成。该方法应有助于更好地理解正常和疾病状态下的肝脏生理学和药理学。
