Akrawi S H, Wedlund P J
J Pharmacol Methods. 1987 Mar;17(1):67-74. doi: 10.1016/0160-5402(87)90038-6.
Surgical procedures for the chronic cannulation of the portal and the femoral veins in the rat are described. The surgical procedure for portal vein cannulation is delicate and requires practice, but has proven useful for chronic drug administration. Cannulation of the femoral vein was less involved and cannula patency was extended by proper cannula preparation and placement. This dual cannulation of the rat allowed infusion of racemic mephenytoin into the portal vein and blood collection from the femoral vein for characterizing blood concentrations of the individual isomers. Chronic portal vein drug infusions may provide a useful approach to studies of time-dependent change in hepatic metabolism and stereoselective first-pass drug elimination in unrestrained animals.
本文描述了大鼠门静脉和股静脉慢性插管的手术方法。门静脉插管的手术操作精细，需要练习，但已证明对慢性给药很有用。股静脉插管相对简单，通过适当的插管准备和放置可延长插管通畅时间。对大鼠进行这种双重插管可将外消旋美芬妥英注入门静脉，并从股静脉采集血液，以测定各对映体的血药浓度。慢性门静脉给药可能为研究自由活动动物肝脏代谢的时间依赖性变化和立体选择性首过药物消除提供一种有用的方法。
