el-Hamzawy M A, Costlow M E
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Anal Biochem. 1988 Jun;171(2):300-4. doi: 10.1016/0003-2697(88)90490-3.
Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.
通过在经聚乙烯亚胺预处理的三醋酸纤维素膜过滤器上过滤，可将游离的125I标记的绵羊催乳素与去污剂溶解的催乳素受体复合物分离。来自大鼠肝脏的高达98%的3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐溶解的催乳素受体复合物与经聚乙烯亚胺处理的膜结合。这种简单快速的技术可用于定量溶解的催乳素受体复合物。
