一种使用聚乙烯亚胺处理的膜滤器对可溶性催乳素受体进行的过滤测定法。

A filtration assay for solubilized prolactin receptors using polyethyleneimine-treated membrane filters.

作者信息

el-Hamzawy M A, Costlow M E

机构信息

Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.

出版信息

Anal Biochem. 1988 Jun;171(2):300-4. doi: 10.1016/0003-2697(88)90490-3.

DOI:10.1016/0003-2697(88)90490-3

PMID:3407927

Abstract

Free 125I-labeled ovine prolactin can be separated from detergent-solubilized prolactin-receptor complex by filtration on triacetate membrane filters pretreated with polyethyleneimine. Up to 98% of the total 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate solubilized prolactin-receptor complexes from rat liver bound to polyethyleneimine-treated membranes. This simple and rapid technique can be used to quantitate solubilized prolactin-receptor complexes.

摘要

通过在经聚乙烯亚胺预处理的三醋酸纤维素膜过滤器上过滤，可将游离的125I标记的绵羊催乳素与去污剂溶解的催乳素受体复合物分离。来自大鼠肝脏的高达98%的3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐溶解的催乳素受体复合物与经聚乙烯亚胺处理的膜结合。这种简单快速的技术可用于定量溶解的催乳素受体复合物。